3.5.2.10: creatininase

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For detailed information about creatininase, go to the full flat file.

Reaction

Synonyms

creatinine amidohydrolase, creatinine hydrolase, CrnA

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.2 In cyclic amides

                3.5.2.10 creatininase

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Results	in table
1	Activating Compound
2108	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
6	Crystallization (Commentary)
5	Disease/ Diagnostics
1	General Stability
19	Inhibitors
8	kcat/KM [mM/s]
13	KM Value [mM]
13	Metals/Ions
10	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
18	Organism
1	Oxidation Stability
5	Pathway
72	PDB ID
5	pH Optimum
4	pH Range
3	pH Stability
1	Posttranslational Modification
9	Protein Variants
5	Purification (Commentary)
4	Reaction
1	Reaction Type
17	Reference
1	Renatured (Commentary)
2	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
19	Substrates and Products (Substrate)
8	Subunits
3	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
4	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 3.5.2.10 - creatininase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Pseudomonas putida

-

677307

ammonium sulfate fractionation, DEAE Sepharose CL-6B and Sepharose CL-6B column chromatographies, 50% total activity

Pseudomonas putida

-

172078

heating, precipitation of nucleic acids with streptomycin, ammonium sulfate precipitation, Sephadex gel filtration, and DEAE-cellulose column chromatography

Paenarthrobacter ureafaciens

-

172083

native and Mn2+-activated enzymes purified, Toyopearl HW65C column and DEAE-Toyopearl column

Pseudomonas putida

P83772

652937

purified by column chromarographies on sarcosine-HM-Sepharose and DEAE-cellulose, and gel filtration on Sephadex G-200

Pseudomonas putida

-

172079