3.5.2.10: creatininase
This is an abbreviated version!
For detailed information about creatininase, go to the full flat file.
Word Map on EC 3.5.2.10
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3.5.2.10
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sarcosine
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creatinase
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amidinohydrolase
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amperometric
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jaffe
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analysis
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medicine
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diagnostics
- 3.5.2.10
- sarcosine
- creatinase
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amidinohydrolase
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amperometric
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jaffe
- analysis
- medicine
- diagnostics
Reaction
Synonyms
creatinine amidohydrolase, creatinine hydrolase, CrnA
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Application
Application on EC 3.5.2.10 - creatininase
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analysis
diagnostics
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the immobilized enzyme can be used in a biosensor for determining creatinine in human serum
medicine
analysis
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construction of a biosensor by co-immobilization of creatininase, creatinase, and sarcosine oxidase onto iron oxide nanoparticles/chitosangraft-polyaniline, Fe3O4-NPs/CHIT-g-PANI, composite film electrodeposited on surface of Pt electrode through glutaraldehyde coupling. The creatinine biosensor uses enzymes/Fe3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The biosensor exhibits an optimum response within 2 s at pH 7.5 and 30°C, when polarized at 0.4 V versus Ag/AgCl. The electrocatalytic response shows a linear dependence on creatinine concentration ranging from 1 to 800 microM. The sensitivity of the biosensor is 3.9 microA per microM and cm2, with a detection limit of 1 microM. The biosensor shows only 10% loss in its initial response after 120 uses over 200 days, when stored at 4°C. The biosensor measures creatinine in the serum of apparently healthy persons