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3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase

This is an abbreviated version!
For detailed information about glutaryl-7-aminocephalosporanic-acid acylase, go to the full flat file.

Word Map on EC 3.5.1.93

Reaction

(7R)-7-(4-carboxybutanamido)cephalosporanate
+
H2O
=
(7R)-7-aminocephalosporanate
+
Glutarate

Synonyms

7beta-(4-carboxybutanamido)cephalosporanic acid acylase, AcyI, acylase ACY 1 proenzyme, adipyl-cephalosporin acylase, antibiotic acylase, CA, CCA, cephalorin acylase, cephalosporin acylase, cephalosporin acylase II, cephalosporin C acylase, cephalosporin-C acylase, CephC acylase, class III acylase, class III GA, class III glutaryl acylase, CPC acylase, CPCAcy, GA, GAR, GCA, GL-7-ACA acylase, GL-7ACA acylase, Gl7ACA acylase, GLA, glutaryl 7-amino cephalosporanic acid acylase, glutaryl 7-aminocephalosporanic acid acylase, glutaryl acylase, glutaryl-7-(7-aminocephalosporanic acid) acylase, glutaryl-7-ACA acylase, glutaryl-7-amino cephalosporanic acid acylase, glutaryl-7-aminocephalosporanic acid acylase, glutaryl-7-aminocephalosporic acid acylase, J1 acylase, N176 cephalosporin C acylase, sCPCAcy, VAC, VAC acylase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylase

pH Stability

pH Stability on EC 3.5.1.93 - glutaryl-7-aminocephalosporanic-acid acylase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
37°C, 30 min, about 75% loss of activity
661368
4 - 6.5
-
37°C, 30 min, stable
661368
5.5
the enzyme activities of both free and immobilized enzymes are greatly reduced at pH 5.5
733139
6 - 8.5
the purified wild-type enzyme is not stable, stability is increased by immobilization
756210
7
37°C, 125 min, 10% loss of wild-type activity, 7% loss of activity of mutant enzyme K198betaA
660767
7 - 11
immobilized GLA shows good stability at pH value below 11.0
686453
7 - 8
-
both forms of the enzyme, free and immobilized are partly inactivated between pH 7 and 8, while the immobilized CPC acylase shows good stability in solutions at pH 8-9.5
713590
8
37°C, 125 min, 13% loss of wild-type activity, 7% loss of activity of mutant enzyme K198betaA
660767
8 - 9.5
9
37°C, 125 min, 20% loss of wild-type activity, 3% loss of activity of mutant enzyme K198betaA
660767
additional information
-
the stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is significantly improved over its free form at lower pH values
660933