3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase
This is an abbreviated version!
For detailed information about glutaryl-7-aminocephalosporanic-acid acylase, go to the full flat file.
Word Map on EC 3.5.1.93
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3.5.1.93
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penicillin
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semisynthetic
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acylases
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deacylation
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d-amino
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synthesis
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autoproteolytic
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beta-lactam
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diminuta
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acremonium
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7-adca
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alphabeta2
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industry
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biotechnology
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pharmacology
- 3.5.1.93
- penicillin
-
semisynthetic
- acylases
-
deacylation
-
d-amino
- synthesis
-
autoproteolytic
- beta-lactam
- diminuta
- acremonium
-
7-adca
-
alphabeta2
- industry
- biotechnology
- pharmacology
Reaction
Synonyms
7beta-(4-carboxybutanamido)cephalosporanic acid acylase, AcyI, acylase ACY 1 proenzyme, adipyl-cephalosporin acylase, antibiotic acylase, CA, CCA, cephalorin acylase, cephalosporin acylase, cephalosporin acylase II, cephalosporin C acylase, cephalosporin-C acylase, CephC acylase, class III acylase, class III GA, class III glutaryl acylase, CPC acylase, CPCAcy, GA, GAR, GCA, GL-7-ACA acylase, GL-7ACA acylase, Gl7ACA acylase, GLA, glutaryl 7-amino cephalosporanic acid acylase, glutaryl 7-aminocephalosporanic acid acylase, glutaryl acylase, glutaryl-7-(7-aminocephalosporanic acid) acylase, glutaryl-7-ACA acylase, glutaryl-7-amino cephalosporanic acid acylase, glutaryl-7-aminocephalosporanic acid acylase, glutaryl-7-aminocephalosporic acid acylase, J1 acylase, N176 cephalosporin C acylase, sCPCAcy, VAC, VAC acylase
ECTree
3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.5.1.93 - glutaryl-7-aminocephalosporanic-acid acylase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
10 - 37
purified recombinant enzyme, the immobilized enzyme CCA is more labile at 37°C than at 20°C or 10°C
15 - 40
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the LX-1000EP-immobilized enzyme is stable at temperatures up to 40°C (8% loss of activity after 2 h of incubation at 40°C)
25
37
40
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immobilized enzyme, 92% activity remains, the free enzyme is nearly inactive
45
50
55
60
additional information
25
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there is no fatal decrease of the enzyme stability up to 25°C. Above 25°C, the stability of the free enzyme decays sharply
37
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1 h, soluble enzyme and enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane are stable, t1/2 is 307.7 min for the soluble enzyme and 733.7 min for the immobilized enzyme
37
half-life: 68.1 h (wild-type enzyme), 88.3 h (mutant enzyme R121betaA), 107.5 h (mutant enzyme K198betaA), 53.9 h (mutant enzyme D286betA)
37
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60 min, soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization lose about 10% of its activity
37
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pH 8.0, t1/2: 600 min for wild-type enzyme, 51 min for beta-subunit mutant enzyme W4A, 98 min for beta-subunit mutant enzyme W4L, 12 min for beta-subunit mutant enzyme W4T, 16 min for beta-subunit mutant enzyme W4F, 307 min for beta-subunit mutant enzyme W4H, 50 min for beta-subunit mutant enzyme W4Y
45
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t1/2 is 49.97 min for the soluble enzyme and 200 min for the immobilized enzyme
45
free wild-type and mutant enzymes: pH 8.5, inactivation after 1 h, immobilized wild-type and mutant enzymes: pH 8.5, 30% and 10% activity remaing after 4 h
45
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pH 7.0, complete inactivation of soluble enzyme after 2 h, complete inactivation of enzyme immobilized on CNBr activated agarose and of enzyme immobilized on glyoxyl agarose, 35% inactivation of enzyme immobilized on amino-epoxy Sepabeads, 60% inactivation of enzyme immobilized on glutaraldehyde activated agarose, 70% inactivation of enzyme immobilized on epoxy Sepabeads
50
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1 h, less than 10% loss of activity at pH 7.0, about 20% less of activity at pH 5, about 35% loss of activity at pH 7, about 70% loss of activity at pH 8, about 90% loss of activity at pH 9
50
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1 h, stable at pH 9.0, about 45% less of activity at pH 6, about 15% loss of activity at pH 7, about 10% loss of activity at pH 8, about 60% loss of activity at pH 10
50
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1 h, less than 10% loss of activity at pH 6.0, about 60% less of activity at pH 5, about 35% loss of activity at pH 7, about 50% loss of activity at pH 8, about 90% loss of activity at pH 9
50
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t1/2 is 29.72 min for the soluble enzyme and 65.9 min for the immobilized enzyme
50
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60 min, soluble enzyme loses 77% of its activity, enzyme immobilized on silica gel modified by epoxide silanization loses 60% of its activity
50
the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control. The immobilized enzyme with sodium acetate shows a 31.7% increase in activity and a 25% increase with sodium bicarbonate. The increased stability and activity recovery in the process of immobilization might be attributed to the increase in the enzyme rigidity via binding of inhibitors with CCA at the active sites
55
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1 h, soluble enzyme loses 77% of activity. t1/2 is 17.03 min for the soluble enzyme and 34.74 min for the immobilized enzyme
60
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pH 8.0, 6 h, 25 mM Tris-HCl, 22% inactivation of wild-type enzyme, 26% inactivation of mutant enzyme M164A, 29% inactivation of mutant enzyme M164G, 20% inactivation of mutant enzyme M164L, 29% inactivation of mutant enzyme M164N, 10% inactivation of mutant enzyme M164Q
additional information
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thermal stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is increased significantly compared to that of the soluble enzyme
additional information
single point mutations Asn2betaThr, Asn2betaVal, Cys470betaSer, Leu154betaPhe, and Leu180betaPhe in hydrophobic core regions, and Ala100alphaSer and Ala37betaSer in segment-segment interface regions, increase the Tm by 4.7-19.7°C, while combining these confirmed single mutations increases the Tm by up to 20.5°C. Construction of six multiple-point variants with negative calculated folding free energy changes
