3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase
This is an abbreviated version!
For detailed information about glutaryl-7-aminocephalosporanic-acid acylase, go to the full flat file.
Word Map on EC 3.5.1.93
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3.5.1.93
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penicillin
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semisynthetic
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acylases
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deacylation
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d-amino
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synthesis
-
autoproteolytic
-
beta-lactam
-
diminuta
-
acremonium
-
7-adca
-
alphabeta2
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industry
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biotechnology
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pharmacology
- 3.5.1.93
- penicillin
-
semisynthetic
- acylases
-
deacylation
-
d-amino
- synthesis
-
autoproteolytic
- beta-lactam
- diminuta
- acremonium
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7-adca
-
alphabeta2
- industry
- biotechnology
- pharmacology
Reaction
Synonyms
7beta-(4-carboxybutanamido)cephalosporanic acid acylase, AcyI, acylase ACY 1 proenzyme, adipyl-cephalosporin acylase, antibiotic acylase, CA, CCA, cephalorin acylase, cephalosporin acylase, cephalosporin acylase II, cephalosporin C acylase, cephalosporin-C acylase, CephC acylase, class III acylase, class III GA, class III glutaryl acylase, CPC acylase, CPCAcy, GA, GAR, GCA, GL-7-ACA acylase, GL-7ACA acylase, Gl7ACA acylase, GLA, glutaryl 7-amino cephalosporanic acid acylase, glutaryl 7-aminocephalosporanic acid acylase, glutaryl acylase, glutaryl-7-(7-aminocephalosporanic acid) acylase, glutaryl-7-ACA acylase, glutaryl-7-amino cephalosporanic acid acylase, glutaryl-7-aminocephalosporanic acid acylase, glutaryl-7-aminocephalosporic acid acylase, J1 acylase, N176 cephalosporin C acylase, sCPCAcy, VAC, VAC acylase
ECTree
3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.5.1.93 - glutaryl-7-aminocephalosporanic-acid acylase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
4.5
4-(glutarylamino)benzoic acid
-
at 37°C, pH not specified in the publication
6.4
glutaryl-gamma-Glu-Cys-Gly
-
at 37°C, pH not specified in the publication
0.041
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, free mutant enzyme
0.043
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, free wild-type enzyme
0.0438
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, immobilized mutant enzyme
0.0484
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, immobilized wild-type enzyme
0.4
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C
0.41
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C
0.6
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C
1.5
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant wild-type enzyme, pH 8.0, 25°C
1.5
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant wild-type enzyme, pH 8.0, 25°C
1.8
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L176betaT, pH 8.0, 37°C
2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/F72betaR, pH 8.0, 37°C
2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L154betaY, pH 8.0, 37°C
3.2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L176betaQ, pH 8.0, 37°C
3.6
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/V25betaR, pH 8.0, 37°C
3.8
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L175betaT, pH 8.0, 37°C
6.3
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/F58betaI, pH 8.0, 37°C
6.6
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L154betaY/M165alphaS, pH 8.0, 37°C
6.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS, pH 8.0, 25°C
9.2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/R24betaP, pH 8.0, 37°C
21.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/M165alphaS, pH 8.0, 37°C
21.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant M165alphaS/H57betaS/H70betaS, pH 8.0, 25°C
0.16
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375G
0.17
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375C
0.51
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375A
0.65
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375M
0.7
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375L
0.7
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Y
0.83
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375N
0.9
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375H
0.9
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375T
0.99
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375S
1
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, wild-type enzyme
1.37
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375V
1.5
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375W
2.07
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375I
2.1
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Q
2.16
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375K
4.3
adipyl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
4.4
adipyl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
7.5
adipyl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
10
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375R
11
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375D
12
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375E
0.14
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266H
0.33
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266M
0.59
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266W
0.6
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266F
0.62
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266Q
0.65
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266Y
0.8
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266C
0.99
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266S
1.4
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266G
2.2
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266A
2.2
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266D
4.4
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266P
5.7
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266T
13.8
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266E
0.14
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266H
0.17
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266Q/F375L
0.17
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266M
0.19
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266H
0.28
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266Q/F375L
0.42
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266S
0.52
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme F229L
0.7
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S
0.7
adipyl-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
0.82
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme F375L
0.96
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, wild-type enzyme
3.8
adipyl-7-aminodesacetoxycephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
7.9
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
1.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C
1.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L176betaC, pH 8.0, 37°C
1.8
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C
2
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/R24betaP, pH 8.0, 37°C
2.3
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/V25betaR, pH 8.0, 37°C
3.1
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L154betaY/M165alphaS, pH 8.0, 37°C
3.8
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L175betaT, pH 8.0, 37°C
4.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L154betaY, pH 8.0, 37°C
4.5
cephalosporin C
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY
5.4
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L176betaT, pH 8.0, 37°C
7.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C
8.1
cephalosporin C
recombinant enzyme mutant M6, pH and temperature not specified in the publication
9.3
cephalosporin C
recombinant mutant L154betaF/L180betaF, pH 8.0, 37°C
9.5
cephalosporin C
pH 8.0, 25°C, recombinant wild-type enzyme
9.73
cephalosporin C
recombinant mutant L154betaF, pH 8.0, 37°C
10.14
cephalosporin C
recombinant mutant L180betaF, pH 8.0, 37°C
11
cephalosporin C
recombinant wild-type enzyme, pH and temperature not specified in the publication
12.2
cephalosporin C
-
recombinant mutant H57betaS/H70betaS, pH 8.0, 25°C
12.2
cephalosporin C
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS
12.38
cephalosporin C
recombinant mutant M31betaF/H57betaS/V68betaA/H70betaS, pH 8.0, 37°C
12.65
cephalosporin C
recombinant mutant M31betaF/H57betaS/H70betaS, pH 8.0, 37°C
16.4
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/F72betaR, pH 8.0, 37°C
16.4
cephalosporin C
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR
27.7
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/M165alphaS, pH 8.0, 37°C
27.7
cephalosporin C
-
recombinant mutant M165alphaS/H57betaS/H70betaS, pH 8.0, 25°C
33.7
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/F58betaI, pH 8.0, 37°C
37
cephalosporin C
mutant enzyme L677F, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
43
cephalosporin C
wild type enzyme, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
86
cephalosporin C
mutant enzyme P295A, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
105
cephalosporin C
mutant enzyme A675G, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
121
cephalosporin C
mutant enzyme H309V, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
1
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
2.2
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
8.7
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
0.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296S
0.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215F
0.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y/H309S
1.1
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme E89A/A215Y
1.2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215V
1.2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y271F
1.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215L
1.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y
1.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme D416Y
1.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296F
2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y215Y/F270S
2.5
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S
2.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme F270M
2.8
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296N
4
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S
4.3
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H417Y
4.6
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H309S
5.5
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296S/H417M
6.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296S/H309S
9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme D416Y/H417Y
25
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y
37.2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y
0.016
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266H
0.031
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, wild-type enzyme
0.033
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, wild-type enzyme
0.038
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375C
0.043
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S
0.048
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme F229L
0.066
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375G
0.07
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375A
0.07
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375S
0.08
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Y
0.09
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375T
0.094
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266S
0.1
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375I
0.1
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375V
0.11
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375H
0.15
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126F
0.17
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375M
0.18
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375N
0.19
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
0.2
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375E
0.21
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
0.22
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375D
0.26
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375L
0.27
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme F375L
0.33
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4A
0.33
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4Y
0.36
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4T
0.37
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4F
0.37
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4L
0.4
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4H
0.5
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, wild-type enzyme
0.5
glutaryl-7-aminocephalosporanic acid
-
at 37°C, pH not specified in the publication
0.6
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375R
0.7
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375K
0.7
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Q
0.8
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375W
0.82
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme H23Q
1.18
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455Q
1.2
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme W173A
1.24
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455D
1.5
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant wild-type enzyme
1.5
glutaryl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
1.69
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126A
1.76
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme H23D
2
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR
2
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY
2.5
glutaryl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
3
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
3.1
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunit mutant enzyme E455L
3.3
glutaryl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
5.4
glutaryl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
6.9
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
6.9
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS
11.7
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme K198A
12.2
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme R121A
12.7
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme D286A
13.4
glutaryl-7-aminocephalosporanic acid
mutant enzyme with mutation Y151F in alpha-subunit and mutation Q50N in beta-subunit
14.4
glutaryl-7-aminocephalosporanic acid
alpha-subunit mutant enzyme Y151F
15.4
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane
17.8
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme Q50N/K198A
17.9
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme Q50N
1.3
glutaryl-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
1.5
glutaryl-7-aminodesacetoxycephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
14.1
glutaryl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
16.1
pentanoyl-7-aminocephalosporanic acid
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mutant enzyme H296S/H309S, at pH 8.0 and 25°C
17.4
pentanoyl-7-aminocephalosporanic acid
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wild type enzyme, at pH 8.0 and 25°C
2.8
succinyl-7-aminocephalosporanic acid
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recombinant enzyme, at pH 8.0 and 25°C
2.8
succinyl-7-aminocephalosporanic acid
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wild type enzyme, at pH 8.0 and 25°C
3.2
succinyl-7-aminocephalosporanic acid
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at 37°C, pH not specified in the publication
5.4
succinyl-7-aminocephalosporanic acid
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mutant enzyme H296S/H309S, at pH 8.0 and 25°C
additional information
7-aminocephalosporanic acid
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estimated value: 9.43 mg/ml, pH 7.6, 34°C, Vmax: 7.65 U/ml
additional information
additional information
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the apparent Km value of CPC acylase increases and Vmax is almost unaffected in the presence of 7-aminocephalosporanic acid
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additional information
additional information
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kinetics of wild-type and mutant enzymes with cephalosporin C, overview
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additional information
additional information
Michaelis-Menten kinetics of free and immobilized wild-type and mutant enzymes, overview
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additional information
additional information
Michaelis constant (Km) and maximum reaction velocity (Vmax) for the free and immobilized enzyme are calculated according to Lineweaver-Burk plots
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additional information
additional information
substrate binding to the protein surface follows a Langmuir model resulting in binding constants K = 7.4 and 9.2 mM for wild-type and mutant M6 enzymes, respectively, which are similar to the experimentally determined Michaelis constants, thermodynamics, overview
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