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3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase

This is an abbreviated version!
For detailed information about glutaryl-7-aminocephalosporanic-acid acylase, go to the full flat file.

Word Map on EC 3.5.1.93

Reaction

(7R)-7-(4-carboxybutanamido)cephalosporanate
+
H2O
=
(7R)-7-aminocephalosporanate
 +
Glutarate

Synonyms

7beta-(4-carboxybutanamido)cephalosporanic acid acylase, AcyI, acylase ACY 1 proenzyme, adipyl-cephalosporin acylase, antibiotic acylase, CA, CCA, cephalorin acylase, cephalosporin acylase, cephalosporin acylase II, cephalosporin C acylase, cephalosporin-C acylase, CephC acylase, class III acylase, class III GA, class III glutaryl acylase, CPC acylase, CPCAcy, GA, GAR, GCA, GL-7-ACA acylase, GL-7ACA acylase, Gl7ACA acylase, GLA, glutaryl 7-amino cephalosporanic acid acylase, glutaryl 7-aminocephalosporanic acid acylase, glutaryl acylase, glutaryl-7-(7-aminocephalosporanic acid) acylase, glutaryl-7-ACA acylase, glutaryl-7-amino cephalosporanic acid acylase, glutaryl-7-aminocephalosporanic acid acylase, glutaryl-7-aminocephalosporic acid acylase, J1 acylase, N176 cephalosporin C acylase, sCPCAcy, VAC, VAC acylase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylase

Inhibitors

Inhibitors on EC 3.5.1.93 - glutaryl-7-aminocephalosporanic-acid acylase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(7R)-7-(4-carboxybutanamido)cephalosporanate
(7R)-7-aminocephalosporanate
-
product inhibition
6-aminopenicillanic acid
6-APA, a noncompetitive inhibitor
7-aminocephalosporanate
7-aminocephalosporanic acid
-
product inhibition, competitive inhibitor
7beta-3-bromopropionyl aminocephalosporanic acid
-
Trp4 of the beta-subunit is alkylated
7beta-bromoacetyl amino cephalosporanic acid
-
inhibits and alkylates the enzyme. The enzyme labeled with 7beta-bromoacetyl amino cephalosporanic acid is inactive at room temperature, but in the process of crystallization at 18°C it catalyzes the hydrolysis of 7beta-bromoacetyl amino cephalosporanic acid.. In crystals, 7-aminocephalosporanic acid is released but the acetic acid still binds with Trp-beta4, and as a result, the enzyme remains inactive
7beta-bromoacetyl aminocephalosporanic acid
-
Trp4 of the beta-subunit is alkylated
cephalosporin C
EDTA
-
5 mM, 27% inhibition
Glutarate
Glutaric acid
glutaryl-7-aminocephalosporanic acid
succinyl-7-aminocephalosporanic acid
-
20 mM, 23% loss of activity
additional information
-