3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase

This is an abbreviated version!
For detailed information about glutaryl-7-aminocephalosporanic-acid acylase, go to the full flat file.

Reaction

Synonyms

7beta-(4-carboxybutanamido)cephalosporanic acid acylase, AcyI, acylase ACY 1 proenzyme, adipyl-cephalosporin acylase, antibiotic acylase, CA, CCA, cephalorin acylase, cephalosporin acylase, cephalosporin acylase II, cephalosporin C acylase, cephalosporin-C acylase, CephC acylase, class III acylase, class III GA, class III glutaryl acylase, CPC acylase, CPCAcy, GA, GAR, GCA, GL-7-ACA acylase, GL-7ACA acylase, Gl7ACA acylase, GLA, glutaryl 7-amino cephalosporanic acid acylase, glutaryl 7-aminocephalosporanic acid acylase, glutaryl acylase, glutaryl-7-(7-aminocephalosporanic acid) acylase, glutaryl-7-ACA acylase, glutaryl-7-amino cephalosporanic acid acylase, glutaryl-7-aminocephalosporanic acid acylase, glutaryl-7-aminocephalosporic acid acylase, J1 acylase, N176 cephalosporin C acylase, sCPCAcy, VAC, VAC acylase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
15	Activating Compound
90	AA Sequence
21	Application
1	CAS Registry Number
33	Cloned(Commentary)
10	Crystallization (Commentary)
12	General Information
8	General Stability
41	Inhibitors
21	kcat/KM [mM/s]
30	Ki Value [mM]
326	KM Value [mM]
2	Localization
36	Molecular Weight [Da]
25	Natural Substrates/ Products (Substrates)
130	Organism
2	Pathway
12	PDB ID
38	pH Optimum
14	pH Range
14	pH Stability
6	pI Value
7	Posttranslational Modification
336	Protein Variants
35	Purification (Commentary)
1	Reaction
3	Reaction Type
111	Reference
27	Specific Activity [micromol/min/mg]
1	Storage Stability
267	Substrates and Products (Substrate)
32	Subunits
172	Synonyms
1	Systematic Name
36	Temperature Optimum [°C]
9	Temperature Range [°C]
28	Temperature Stability [°C]
160	Turnover Number [1/s]

General Stability

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

General Stability on EC 3.5.1.93 - glutaryl-7-aminocephalosporanic-acid acylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to General Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

45°C, pH 7.0, complete inactivation of soluble enzyme after 2 h, complete inactivation of enzyme immobilized on CNBr activated agarose and of enzyme immobilized on glyoxyl agarose, 35% inactivation of enzyme immobilized on amino-epoxy Sepabeads, 60% inactivation of enzyme immobilized on glutaraldehyde activated agarose, 70% inactivation of enzyme immobilized on epoxy Sepabeads

unidentified

-

662487

Eupergit C-immobilized acylase has a half-life of greater than 16 days

Pseudomonas sp.

-

660734

FP-IDA-Ni2+ resins-immobilized GLA is recycled 21times without significant loss of activity

Pseudomonas sp.

O86089

686453

immobilized mutant enzyme M269Y/C305S is effective in more than 60 cycles for the production of 7-aminocephalosporanate

Pseudomonas sp.

-

660711

in the results of repeated batch production of 7-aminocephalosporanic acid, 50% activity of the initial cycle is maintained after recycled 24 times and the average conversion rate of cephalosporin reaches 98%

Pseudomonas sp. SE83

-

713590

maltose-binding protein improves the solubility of the enzyme

Pseudomonas sp.

-

686445

the enzyme is thermostabilized by immobilization using epoxide silanization

Pseudomonas sp.

-

671010

the stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is significantly improved over its free form at lower pH values

Pseudomonas sp.

-

660933