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biotechnology
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a biological and colometric method is evaluated for determination of cephalosporin acylase product in bacteria
biotechnology
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with an aim to increase the yield of 7-aminocephalosporanic acid production, a stepwise strategy, statistical medium is applied for optimizing the medium composition for the production of CPC acylase
industry
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great potential for industrial application of the lysis genes-assisted cell disruption
industry
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the huge production of engineered GLA produced in Escherichia coli under optimized conditions makes this enzyme an economic tool to be used in bioconversion processes at the industrial level, e.g., in 7-ACA production
industry
cephalosporin C acylase (CCA), a proton-forming enzyme, is an important industrial enzyme that can directly catalyze the substrate cephalosporin C (CPC) to 7-aminocephalosporanic acid (7-ACA), which is an intermediate in many types of synthetic cephalosporins. Immobilized CCA on porous carriers is applied in industry for the production of 7-ACA because of its high efficiency and environmentally friendly nature compared with the chemical process and two-step enzymatic process involving two enzymes. Mutational improvement of catalytic efficiency and operational stability
industry
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great potential for industrial application of the lysis genes-assisted cell disruption
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pharmacology
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the product of the reaction 7-aminocephalosporanic acid is a starting material for semisynthetic cephalosporin antibiotics. High thermal stability of the enzyme immobilized on silica gels indicates that it can be successfully used for the production of 7-aminocephalosporanic acid on an industrial scale
pharmacology
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the product of the reaction 7-aminocephalosporanic acid is a starting material for semisynthetic cephalosporin antibiotics. High thermal stability of the enzyme immobilized on silica gels indicates that it can be successfully used for the production of 7-aminocephalosporanic acid on an industrial scale
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synthesis
enzymatic production of 7-aminocephalosporanate. Construction of site-directed mutants with enhanced activity and stability
synthesis
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immobilized mutant enzyme M269Y/C305S may be a promising enzyme in one-step enzymatic production of 7-aminocephalosporanate from cephalosporin C
synthesis
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mutant enzyme M269Y may be a promising enzyme in the one-step enzymic production of 7-amino-cephalosporanic acid from cephalosporin C
synthesis
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bioconversion of cephalosporin C into 7-aminocephalosporanate
synthesis
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D-amino acid oxidase in the permeabilized Pichia pastoris cells and immobilized glutaryl-7-aminocephalosporanic acid acid acylase on support, are employed to convert cephalosporin to 7-aminocephalosporanic acid in a single reactor
synthesis
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production of 7-aminocephalosporanate on a large scale at 25°C using enzyme immobilized by epoxide silanization
synthesis
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immobilzation on LX-1000EP, an epoxy support, with a recovery of 33.8% and an activity of 81 U/g wet resin, suggesting a potential in industrial 7-aminocephalosporanic acid production. Under optimal conditions, a very high 7-aminocephalosporanic acid yield of 96.6% is obtained within 60 min
synthesis
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the enzyme mutant may be suitable for industrial application of the mono-step process for cephalosporin C conversion to (7R)-7-aminocephalosporanate, which is the precursor for production of semisynthetic cephalosporins
synthesis
antibiotic acylases are key enzymes for the industrial production of antibiotic drugs
synthesis
biocatalytic synthesis by the cephalosporin acylase from Pseudomonas sp. strain N176 is a promising alternative to chemical semisynthesis of cephalosporins antibiotics
synthesis
cephalosporin C acylase (CCA), a proton-forming enzyme, is an important industrial enzyme that can directly catalyze the substrate cephalosporin C (CPC) to 7-aminocephalosporanic acid (7-ACA), which is an intermediate in many types of synthetic cephalosporins. Immobilized CCA on porous carriers is applied in industry for the production of 7-ACA because of its high efficiency and environmentally friendly nature compared with the chemical process and two-step enzymatic process involving two enzymes
synthesis
the enzyme is used for production of the important cephalosporin antibiotic 7-aminocephalosporanic acid (7-ACA). 7-ACA is an important cephalosporin nucleus for the synthesis of many widely used beta-lactam antibiotics
synthesis
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production of 7-aminocephalosporanate on a large scale at 25°C using enzyme immobilized by epoxide silanization
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