3.5.1.86: mandelamide amidase

This is an abbreviated version!
For detailed information about mandelamide amidase, go to the full flat file.

Reaction

Synonyms

amidase, mandelamide, MAH, mandelamide hydrolase, Pseudomonas mandelamide hydrolase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.86 mandelamide amidase

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Results	in table
291	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
1	General Information
9	Inhibitors
5	kcat/KM [mM/s]
6	Ki Value [mM]
62	KM Value [mM]
7	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
16	Organism
2	Pathway
3	pH Optimum
20	Protein Variants
5	Purification (Commentary)
1	Reaction
1	Reaction Type
15	Reference
1	Specific Activity [micromol/min/mg]
2	Storage Stability
45	Substrates and Products (Substrate)
2	Subunits
9	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
52	Turnover Number [1/s]

Reference

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Reference on EC 3.5.1.86 - mandelamide amidase

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288942

Yamamoto, K.; Oishi, K.; Fujimatsu, I.; Komatsu, K.

Production of R-(-)-mandelic acid from mandelonitrile by Alcaligenes faecalis ATCC 8750

Appl. Environ. Microbiol.

3028-3032

1991

Alcaligenes faecalis, Brevundimonas vesicularis, Meyerozyma guilliermondii, Corynebacterium nitrilophilus, Corynebacterium sp., Mycobacterium sp., Rhodococcus sp., Corynebacterium sp. C5, Mycobacterium sp. AC777, Rhodococcus sp. AK32

1660699

288943

Kooo, C.W.

Mandelamide hydrolase from Pseudomonas putida

Diss. Abstr. Int. B

1865

1997

Pseudomonas putida

650254

Gopalakrishna, K.N.; Stewart, B.H.; Kneen, M.M.; Andricopulo, A.D.; Kenyon, G.L.; McLeish, M.J.

Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family

Biochemistry

7725-7735

2004

Pseudomonas putida (Q84DC4), Pseudomonas putida

15196015

680440

McLeish, M.J.; Kneen, M.M.; Gopalakrishna, K.N.; Koo, C.W.; Babbitt, P.C.; Gerlt, J.A.; Kenyon, G.L.

Identification and characterization of a mandelamide hydrolase and an NAD(P)+dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633

J. Bacteriol.

185

2451-2456

2003

Pseudomonas putida

12670968

694955

Wang, P.F.; Yep, A.; Kenyon, G.L.; McLeish, M.J.

Using directed evolution to probe the substrate specificity of mandelamide hydrolase

Protein Eng. Des. Sel.

103-110

2009

Pseudomonas putida

19074156

755893

Adediran, S.A.; Wang, P.F.; Shilabin, A.G.; Baron, C.A.; McLeish, M.J.; Pratt, R.F.

Specificity and mechanism of mandelamide hydrolase catalysis

Arch. Biochem. Biophys.

618

23-31

2017

Pseudomonas putida (Q84DC4)

28129982