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3.5.1.86: mandelamide amidase

This is an abbreviated version!
For detailed information about mandelamide amidase, go to the full flat file.

Reaction

(R)-mandelamide
+
H2O
=
(R)-mandelate
+
NH3

Synonyms

amidase, mandelamide, MAH, mandelamide hydrolase, Pseudomonas mandelamide hydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.86 mandelamide amidase

Purification

Purification on EC 3.5.1.86 - mandelamide amidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
recombinant enzyme
Sephacryl S-200 gel filtration, HiPrep Q anion-exchange column chromatography, and HiTrap Q column chromatography
-