3.5.1.86: mandelamide amidase
This is an abbreviated version!
For detailed information about mandelamide amidase, go to the full flat file.
Reaction
Synonyms
amidase, mandelamide, MAH, mandelamide hydrolase, Pseudomonas mandelamide hydrolase
ECTree
3.5.1.86 mandelamide amidaseAdvanced search results
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results (Engineering
Engineering on EC 3.5.1.86 - mandelamide amidase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
F150L
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type
F433A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type
F433L
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type
G202A
-
site-directed mutagenesis. Gly202 appears to control the preference for aromatic substrates as the G202A variant shows three orders of magnitude decrease in kcat/Km for (R)- and (S)-mandelamide
G202V
-
site-directed mutagenesis. Reduction in activity increases to six orders of magnitude for the G202V variant
K100A
S180A
S204A
K100A
site-directed mutagenesis, mutation of a catalytic residue, inactive mutant
S180A
site-directed mutagenesis, mutation of a catalytic residue, 4200fold decrease in the kcat/Km value
S204A
site-directed mutagenesis, mutation of a catalytic residue, inactive mutant
