3.5.1.81: N-Acyl-D-amino-acid deacylase

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For detailed information about N-Acyl-D-amino-acid deacylase, go to the full flat file.

Reaction

Synonyms

AcyM, AxD-NAase, D-AAase, D-AGase, D-amidohydrolase, D-Amino acylase, D-Aminoacylase, D-ANase, D-NAase, DAA, dan, N-Acyl-D-amino acid amidohydrolase, N-acyl-D-amino-acid deacylase, N-Acyl-D-aspartate amidohydrolase, N-acyl-D-glutamate amidohydrolase, N-D-AAase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.81 N-Acyl-D-amino-acid deacylase

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Results	in table
2	Activating Compound
1320	AA Sequence
16	Application
1	CAS Registry Number
9	Cloned(Commentary)
2	Crystallization (Commentary)
8	General Information
5	General Stability
1	IC50 Value
72	Inhibitors
15	kcat/KM [mM/s]
4	Ki Value [mM]
45	KM Value [mM]
8	Metals/Ions
16	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
69	Organism
8	PDB ID
19	pH Optimum
5	pH Range
21	pH Stability
2	pI Value
51	Protein Variants
16	Purification (Commentary)
1	Reaction
1	Reaction Type
51	Reference
1	Renatured (Commentary)
8	Source Tissue
33	Specific Activity [micromol/min/mg]
2	Storage Stability
199	Substrates and Products (Substrate)
19	Subunits
60	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
4	Temperature Range [°C]
24	Temperature Stability [°C]
25	Turnover Number [1/s]

Cloned

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Cloned on EC 3.5.1.81 - N-Acyl-D-amino-acid deacylase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloning by sequence-based screening, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli and Streptomyces lividans

Streptomyces sp.

S0AUN2

735339

DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis

Microbacterium natoriense

I7HFV7

734334

expression in Escherichia coli

Achromobacter xylosoxidans

-

31912

expression of wild-type and mutant enzymes in Escherichia coli

Achromobacter xylosoxidans

-

670809

gene ADdan, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) using pET-28a with a T7 promoter

Achromobacter denitrificans

S4T8N6

734635

ovoerproduction in Escherichia coli

Achromobacter xylosoxidans

-

31913

recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109

Variovorax paradoxus

Q6EMR8

735200

three isozymes D-ANase, D-AGase, and D-AAase, soluble expression of the enzyme in Escherichia coli facilitated by molecular chaperones, overview

Achromobacter xylosoxidans

-

669735

wild type and mutant enzymes are expressed in Escherichia coli JM109 cells

Achromobacter xylosoxidans

-

720103