3.5.1.75: urethanase

This is an abbreviated version!
For detailed information about urethanase, go to the full flat file.

hydrolase, urethane, More, UreC, urethane hydrolase, urethane-hydrolyzing enzyme

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Results	in table
2	Activating Compound
2	AA Sequence
17	Application
1	CAS Registry Number
2	Cloned(Commentary)
1	Cofactor
2	General Information
43	Inhibitors
21	KM Value [mM]
7	Localization
10	Metals/Ions
5	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
7	Organic Solvent Stability
47	Organism
1	Oxidation Stability
20	pH Optimum
6	pH Range
8	pH Stability
6	Protein Variants
7	Purification (Commentary)
2	Reaction
1	Reaction Type
27	Reference
1	Renatured (Commentary)
16	Source Tissue
9	Specific Activity [micromol/min/mg]
96	Substrates and Products (Substrate)
10	Subunits
14	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
5	Temperature Range [°C]
12	Temperature Stability [°C]

Purification on EC 3.5.1.75 - urethanase

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native enzyme by ultrasonication, concentration by PEG 20000, gel filtration, and ultrafilatration

native enzyme to homogeneity by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography

native urethanase 3.1fold by ethanol precipitation, and anion exchange chromatography

partial

recombinant His-tagged enzyme, refolded from Escherichia coli strain BL21(DE3) inclusion bodies, 1.22fold by nickel affinity chromatography

strain IFO 12107