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3.5.1.72: D-benzoylarginine-4-nitroanilide amidase

This is an abbreviated version!
For detailed information about D-benzoylarginine-4-nitroanilide amidase, go to the full flat file.

Word Map on EC 3.5.1.72

Reaction

N-benzoyl-D-arginine-4-nitroanilide
+
H2O
=
N-benzoyl-D-arginine
+
4-nitroaniline

Synonyms

amidase, benzoyl-D-arginine aryl-, benzoyl-D-arginine arylamidase, D-BAPA-ase, D-BAPA-hydrolyzing enzyme, D-BAPAase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.72 D-benzoylarginine-4-nitroanilide amidase

Temperature Stability

Temperature Stability on EC 3.5.1.72 - D-benzoylarginine-4-nitroanilide amidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
strain 168, 2 min, 40% loss of activity in 0.05 M Tris buffer, pH 7.8, 60% loss of activity with 5 mM MgCl2, 80% loss of activity with 5 mM CaCl2
additional information
-
strain 168, Ca2+ and Mg2+ ions decrease thermostability