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3.5.1.71: N-feruloylglycine deacylase

This is an abbreviated version!
For detailed information about N-feruloylglycine deacylase, go to the full flat file.

Reaction

N-feruloylglycine
+
H2O
=
ferulate
+
glycine

Synonyms

hydrolase, N-feruloylglycine, N-feruloylglycine hydrolase, N-FGAH

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.71 N-feruloylglycine deacylase

Inhibitors

Inhibitors on EC 3.5.1.71 - N-feruloylglycine deacylase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
o-phenanthroline, 10 mM: 97% inhibition, partially reversed by CuCl2, CoCl2 and to a lesser extent by ZnCl2 and MnCl2, 1 mM: 29% inhibition, 0.1 mM: no inhibition. 2.5 mM: 73% inhibition, totally reversed by 0.66 mM ZnCl2, CuCl2 or CoCl2 and to a lesser extent also by nickel and manganous ions
1,4-dithiothreitol
-
2 mM: 100% inhibition
2-Iodosobenzoate
-
5 mM: 100% inhibition
2-mercaptoethanol
-
20 mM: 100% inhibition
Co2+
-
1 mM: about 37% inhibition
CoSO4
-
1 mM: about 37% inhibition
diphenyl-1,5-thiocarbazone
-
dithizone, 10 mM: 79% inhibition
EDTA
-
10 mM: 61% inhibition
glutathione
-
reduced glutathione, 50 mM: 100% inhibition
HgCl2
-
0.005 mM: 100% inhibition
iodoacetamide
-
7.5 mM: 100% inhibition
iodoacetate
-
4 mM: 100% inhibition
L-cysteine
-
50 mM: 100% inhibition
Metal chelating reagents
-
high concentration, 10 mM
-
N-ethylmaleimide
-
1 mM: 100% inhibition
N-feruloyl-Gly-L-LeuOH
-
5 mM: potent reversible inhibitor, kinetics of inhibition. Ki: 0.3 mM
N-feruloyl-Gly-L-PheOH
-
5 mM: potent reversible inhibitor, kinetics of inhibition. Ki: 0.042 mM
N-feruloyldipeptides
-
potent reversible inhibitor
p-chloromercuribenzoate
-
0.05 mM: 100% inhibition
sulfhydryl reagents
-
-
additional information
-
kinetics of inhibition; no inhibition by cystine and disodium maleate
-