3.5.1.69: glycosphingolipid deacylase
This is an abbreviated version!
For detailed information about glycosphingolipid deacylase, go to the full flat file.
Reaction
Synonyms
deacylase, glycosphingolipid ceramide, glycosphingolipid ceramide deacylase, PS_SCD, SA_SCD, SCDase, sphingolipid ceramide N-acylase, sphingolipid ceramide N-deacylase
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Activating Compound
Activating Compound on EC 3.5.1.69 - glycosphingolipid deacylase
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sodium cholate
activates at 0.07-0.7% w/v, loss of about 75% activation potency in absence of Ca2+
taurodeoxycholate hydrate
TDC, activates at 0.07-0.7% w/v, TDC-based enhancement of hydrolysis requires the presence of Ca2+. In the absence of Ca2+, GM1 hydrolysis is only 2.9%, 14fold lower than the same case of Triton X-100
Tween 80
activates 0.07-0.7% w/v, loss of about 65% activation potency in absence of Ca2+
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Triton X-100
activates at 0.07-0.7% w/v, loss of about 50% activation potency in absence of Ca2+