3.5.1.60: N-(long-chain-acyl)ethanolamine deacylase

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For detailed information about N-(long-chain-acyl)ethanolamine deacylase, go to the full flat file.

Reaction

Synonyms

acylethanolamine acid amidase, amidase, acylethanolamine, hNAAA, N-acylethanolamine acid amidase, N-acylethanolamine amidohydrolase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, Nacylethanolamine acid amidase, NAE-hydrolyzing acid amidase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.60 N-(long-chain-acyl)ethanolamine deacylase

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Results	in table
1	Activating Compound
8	AA Sequence
10	Application
1	CAS Registry Number
19	Cloned(Commentary)
3	Crystallization (Commentary)
30	General Information
1	General Stability
161	IC50 Value
295	Inhibitors
8	Ki Value [mM]
8	KM Value [mM]
30	Localization
6	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
40	Organism
26	PDB ID
17	pH Optimum
1	pH Range
10	Posttranslational Modification
13	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
36	Reference
51	Source Tissue
1	Storage Stability
55	Substrates and Products (Substrate)
8	Subunits
68	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]

Purification

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Purification on EC 3.5.1.60 - N-(long-chain-acyl)ethanolamine deacylase

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Rattus norvegicus

209214

recombinant C-terminally His6-tagged enzyme from HEK-293 cells by nickel affinity chromatography

Homo sapiens

Q02083

735064

recombinant His-tagged enzyme from Spodoptera frugiperda Sf9 insect cells by nickel affinity chromatography, gel filtration, and anion exchange chromatography

Oryctolagus cuniculus

G1T7U7

755270

recombinant His-tagged wild-type and mutant enzymes from Spodoptera frugiperda Sf9 insect cells by nickel affinity chromatography, gel filtration, and anion exchange chromatography

Cavia porcellus

H0VCJ6

755270

recombinant His6-tagged wild-type and mutant enzymes from Spodoptera frugiperda Sf9 insect cells by nickel affinity chromatography, gel filtration, and anion exchange chromatography

2 entries

recombinant His6-tagged zymogen from HEK-293 cells by ammonium sulfate fractionation, dialysis, metal affinity chromatography, and again dialysis followed by ultrafiltration

Homo sapiens

Q02083

734562