3.5.1.60: N-(long-chain-acyl)ethanolamine deacylase
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For detailed information about N-(long-chain-acyl)ethanolamine deacylase, go to the full flat file.
Reaction
Synonyms
acylethanolamine acid amidase, amidase, acylethanolamine, hNAAA, N-acylethanolamine acid amidase, N-acylethanolamine amidohydrolase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, Nacylethanolamine acid amidase, NAE-hydrolyzing acid amidase
ECTree
3.5.1.60 N-(long-chain-acyl)ethanolamine deacylaseAdvanced search results
results (
results (Cloned
Cloned on EC 3.5.1.60 - N-(long-chain-acyl)ethanolamine deacylase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expressed in COS-7 cells
gene NAAA, genotyping, six splice variants (a1, b1, c1, a2, b2, and c2), whereof four major splice variants (a1, a2, b2, and c2) are identified in a human prostate cancer cell line LNCaP, they are composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively, quantitative PCR expression analysis, method development for individual quantification. Variants a1 and a2 encode the same full-length NAAA protein, which is catalytically active, while b2 and c2 are translated to C-terminally truncated proteins. Recombinant expression in HEK-293 cells. The truncated forms are detected as catalytically inactive precursor proteins, but not as mature forms, expression of N-terminally FLAG-tagged isoform A of human NAAA, corresponding to splice variant a1, in HEK-293 cells. The mature enzyme form loses the N-terminal FLAG tag
gene Naaa, stable transfection and functional recombinant expression in HEK-293 cells
recombinant expression of enzyme NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Construct encompasses residues 31-359 of NAAA of mouse enzyme
recombinant expression of the C-terminally His6-tagged enzyme in HEK-293 cells
recombinant expression of the enzyme in HEK-293 cells
recombinant expression of wild-type and mutant enzymes NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide comprising the first 28-33 residues is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Constructs encompassed residues 29-359 of NAAA of human enzyme
recombinant expression of wild-type and mutant enzymes NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Constructs encompass residues 29-355 of NAAA of mouse enzyme
recombinant expression of wild-type and mutant enzymes NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Constructs encompass residues 34-362 of NAAA of mouse enzyme
recombinant overexpression in HEK-293 cell membranes
recombinant stable expression of the His6-tagged zymogen in HEK-293 cells, ammonium chloride treatment stimulates secretion of the lysosomal proteins from the HEK293 cells
