3.5.1.6: beta-ureidopropionase
This is an abbreviated version!
For detailed information about beta-ureidopropionase, go to the full flat file.
Word Map on EC 3.5.1.6
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3.5.1.6
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pyrimidine
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uracil
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dihydropyrimidinase
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thymine
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dihydrouracil
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kluyveri
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nitrilase
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amidohydrolases
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beta-aminoisobutyric
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beta-ureidoisobutyrate
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medicine
- 3.5.1.6
- pyrimidine
- uracil
- dihydropyrimidinase
- thymine
- dihydrouracil
- kluyveri
- nitrilase
-
amidohydrolases
-
beta-aminoisobutyric
- beta-ureidoisobutyrate
- medicine
Reaction
Synonyms
3-ureidopropionase, beta-Ala synthase, beta-alanine synthase, beta-UP, beta-ureidopropionase, beta-ureidopropionate decarbamylase, betaAS, betaUP, betaUPase, BUP-1, BUP1, human liver beta-ureidopropionase, N-carbamoyl-beta-Ala amidohydrolase, N-carbamoyl-beta-alanine amidohydrolase, N-carbamyl-beta-alanine decarbamylase, NCbetaA, PYD3, SkbetaAS, UPB1
ECTree
3.5.1.6 beta-ureidopropionaseAdvanced search results
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Engineering on EC 3.5.1.6 - beta-ureidopropionase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
R291E
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R291 is the key residue for recognition of the substrate carboxyl group
R291K
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R291 is the key residue for recognition of the substrate carboxyl group
R291Q
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R291 is the key residue for recognition of the substrate carboxyl group
R291E
Agrobacterium tumefaciens C58 / ATCC 33970
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R291 is the key residue for recognition of the substrate carboxyl group
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R291K
Agrobacterium tumefaciens C58 / ATCC 33970
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R291 is the key residue for recognition of the substrate carboxyl group
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R291Q
Agrobacterium tumefaciens C58 / ATCC 33970
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R291 is the key residue for recognition of the substrate carboxyl group
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A85E
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expression of the A85E plasmid results in severely reduced BUP-1 enzyme activity, with only 2.7% activity relative to the wild-type UPB1 plasmid
G235R
naturally occuring mutation and site-directed mutagenesis, inactive mutant. Mutation G235R introduces a large amino acid side chain for which there is no space available at this location. The larger structural rearrangements in the active site cavity required to prevent clashes with surrounding residues are expected to lead to enzyme inactivity and misfolding and defects in oligomerization, inability to obtain significant expression of soluble protein for this mutant
L13S
R130D/S208R
inactive mutant enzyme, the mutant enzyme is exclusively dimeric
R130I
inactive mutant enzyme, a significant monomer proportion is detected
R236W
naturally occuring mutation and site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R326Q
S208A
inactive mutant enzyme, a significant monomer proportion is detected
S264R
naturally occuring mutation and site-directed mutagenesis,mutation S264R abolishes the hydrogen bond to Y314, which may be important for structural fixation of a residue stretch that is involved in shaping the entrance to the active site, the mutant shows reduced activity compared to the wild-type enzyme
T359M
additional information
L13S
naturally occuring mutation and site-directed mutagenesis, the mutation results in folding defects and oligomer assembly impairment, the mutant shows reduced activity compared to the wild-type enzyme
L13S
inactive mutant enzyme, the mutation is identified in beta-ureidopropionase-deficient patients
R326Q
naturally occuring mutation and site-directed mutagenesis, the mutation results in folding defects and oligomer assembly impairment, inactive mutant
R326Q
naturally occuring mutation in exon 9 affecting pre-mRNA splicing, analysis using a minigel approach
T359M
naturally occuring mutation and site-directed mutagenesis, the mutation results in folding defects and oligomer assembly impairment, the mutant shows highly reduced activity compared to the wild-type enzyme
additional information
construction of a T-DNA knockout mutant of gene PYD3, the mutant exhibits no obvious phenotype under optimal growing conditions, but the pyd3 mutant is unable to catabolize [2-14C]-uracil or to grow on uracil as the sole nitrogen source
additional information
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screening for genetic deficiency in betaUPase, genotyping and phenotypes, overview
additional information
missense mutations and intronic mutations that lead to aberrant splicing, overview
additional information
disruption of dimer-dimer interfaces by site-directed mutagenesis generated dimeric, inactive enzyme variants
additional information
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transgenic overexpression of SlUPB1 in tomato, upregulation of gene SlUPB1 in heat-sensitive variant L4783 and downregulation of gene SlUPB1 in heat-tolerant variant CL5915. Pollen germination rates of transgenics overexpressing SlUPB1 are higher than germination rates of the background tomato L4783. Germination rates of transgenics downregulating SlUPB1 are significantly lower than germination rates of background tomato CL5915
additional information
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transgenic overexpression of SlUPB1 in tomato, upregulatiopn of gene SlUPB1 in heat-sensitive variant L4783 and downregulation of gene SlUPB1 in heat-tolerant variant CL5915. Pollen germination rates of transgenics overexpressing SlUPB1 are higher than germination rates of the background tomato L4783. Germination rates of transgenics downregulating SlUPB1 are significantly lower than germination rates of background tomato CL5915
additional information
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transgenic overexpression of SlUPB1 in tomato, upregulation of gene SlUPB1 in heat-sensitive variant L4783 and downregulation of gene SlUPB1 in heat-tolerant variant CL5915. Pollen germination rates of transgenics overexpressing SlUPB1 are higher than germination rates of the background tomato L4783. Germination rates of transgenics downregulating SlUPB1 are significantly lower than germination rates of background tomato CL5915
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additional information
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transgenic overexpression of SlUPB1 in tomato, upregulatiopn of gene SlUPB1 in heat-sensitive variant L4783 and downregulation of gene SlUPB1 in heat-tolerant variant CL5915. Pollen germination rates of transgenics overexpressing SlUPB1 are higher than germination rates of the background tomato L4783. Germination rates of transgenics downregulating SlUPB1 are significantly lower than germination rates of background tomato CL5915
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additional information
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transgenic overexpression of SlUPB1 in tomato, upregulation of gene SlUPB1 in heat-sensitive variant L4783 and downregulation of gene SlUPB1 in heat-tolerant variant CL5915. Pollen germination rates of transgenics overexpressing SlUPB1 are higher than germination rates of the background tomato L4783. Germination rates of transgenics downregulating SlUPB1 are significantly lower than germination rates of background tomato CL5915
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additional information
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transgenic overexpression of SlUPB1 in tomato, upregulatiopn of gene SlUPB1 in heat-sensitive variant L4783 and downregulation of gene SlUPB1 in heat-tolerant variant CL5915. Pollen germination rates of transgenics overexpressing SlUPB1 are higher than germination rates of the background tomato L4783. Germination rates of transgenics downregulating SlUPB1 are significantly lower than germination rates of background tomato CL5915
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