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3.5.1.6: beta-ureidopropionase

This is an abbreviated version!
For detailed information about beta-ureidopropionase, go to the full flat file.

Word Map on EC 3.5.1.6

Reaction

3-ureidopropanoate
+
H2O
=
beta-Alanine
 +
CO2
 +
NH3

Synonyms

3-ureidopropionase, beta-Ala synthase, beta-alanine synthase, beta-UP, beta-ureidopropionase, beta-ureidopropionate decarbamylase, betaAS, betaUP, betaUPase, BUP-1, BUP1, human liver beta-ureidopropionase, N-carbamoyl-beta-Ala amidohydrolase, N-carbamoyl-beta-alanine amidohydrolase, N-carbamyl-beta-alanine decarbamylase, NCbetaA, PYD3, SkbetaAS, UPB1

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.6 beta-ureidopropionase

Cloned

Cloned on EC 3.5.1.6 - beta-ureidopropionase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding human beta-ureidopropionase gene, BUP-1 cloned, plasmid yc28d10.r1 sequenced and expressed in Escherichia coli BL21, eukaryotic expression vector pCR3 transfected to COS-7-cells
cDNA truncated at the N-terminus by 14 residues, overexpression in Escherichia coli BL21
-
expression in Escherichia coli B843(DE3)
-
expression in Escherichia coli Rosetta (DE3)pLysS cells
expression of GFP-tagged PYD3 in Arabdiopsis thaliana via Agrobacterium-mediated transformation using the floral dip method. Quantitative real-time RT-PCR analysis of PYD expression in wild-type and pyd mutants, overview
gene SIUPB1, cloning, transgenic overexpression by Agrobacterium tumefaciens-mediated transformation, and expression analysis
-
gene SIUPB1, cloning, transgenic overexpression, and expression analysis
-
gene UPB1, DNA and amino acid sequence determination and analysis, genotyping, reverse transcription and quantitative real-time PCR enzyme expression analysis, overview
gene UPB1, genotyping
-
gene UPB1, located on chromosome 22q11.2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in HEK293 cells
gene UPB1, located on chromosome 22q11.2, DNA and amino acid sequence determination and analysis, sequence comparisons and genomic organization analysis, genotyping of beta-ureidopropionase deficient patients as well as the analysis of the mutations in a three-dimensional framework, PCR enzyme expression analysis, overview. Recombinant heterologous expression of wild-type and mutant enzymes in Escherichia coli strain C41(DE3)pRARE2, all mutations yield mutant beta-ureidopropionase proteins with significantly decreased activity
hexahistidine fusion protein expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL
His-tagged version expressed in Escherichia coli
-
mutation A85E is introduced into the wild-type UPB1 plasmid using site-directed mutagenesis. The mutated plasmid is then transfected into the RKO cell line
-