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3.5.1.54: allophanate hydrolase

This is an abbreviated version!
For detailed information about allophanate hydrolase, go to the full flat file.

Word Map on EC 3.5.1.54

Reaction

urea-1-carboxylate
+
H2O
= 2 CO2 + 2 NH3

Synonyms

allophanate lyase, AtzF, DUR1,2, GbCGDNIH1_1744, KlAH, KLLA0_E08119g, PsAH, TrzF, UAL, urea amidolyase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.54 allophanate hydrolase

Cloned

Cloned on EC 3.5.1.54 - allophanate hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene atzF, DNA and amino acid sequence determination and analysis, recombinant expression of functional His-tagged enzyme in Escherichia coli strain BL21 (lambdaDE3)
gene atzF, expression of the His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene atzF, sequence comparisons, cloning of atzF and the truncated gene encoding the amidase (N-terminal) domain of AtzF (atzF467), recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene atzF, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(lambdaDE3)
gene CGDNIH1, expression of N-terminally His8-tagged wild-type and mutant enzymes in Escherichia coli strain HMS174 (DE3)
gene KLLA0_E08119g, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)Star
gene trzF, overexpression of His-tagged enzyme in presence of a chaperone in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli strain BL21(DE3)
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
recombinant expression of wild-type and mutant His-tagged SeMet-substituted enzymes in Escherichia coli strain BL21 Star(DE3)
sequence analysis and comparison, and phylogenetic analysis