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3.5.1.52: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

This is an abbreviated version!
For detailed information about peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase, go to the full flat file.

Word Map on EC 3.5.1.52

Reaction

Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser
+
H2O
=
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc
 +
Asn-Asp-Glu-Ser-Ser

Synonyms

acid PNGase M, acidic peptide:N-glycanase, acidic PNGase, aPNGase, At3g14920, At5g05480, AtPNG1, DDB0189828, EC 3.2.2.18, glycoamidase, glycopeptidase, glycopeptide N-glycosidase, jackbean glycopeptidase, L-929 PNGase, MPng1, N-glycanase, N-glycosidase F, N-oligosaccharide glycopeptidase, Ncpng1, neutral PNGase M, Ngly1, peptide N-glycanase, peptide N-glycosidase, peptide N-glycosidase F, peptide N4(N-acetyl-glucosaminyl)asparagine amidase, peptide-N-(N-acetyl-beta-glucosaminyl) asparagine amidase F, peptide-N-glycanase, peptide-N-glycosidase F, peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase, peptide: N-glycanase, peptide: N-glycosidase, peptide:N-glycanase, peptide:N-glycanase homolog, peptide:N-glycosidase, peptidyl N-glycanase F, PGNase, PNG-1, PNG1, Png1p, PNGase, PNGase A, PNGase A-like enzyme, PNGase At, PNGase F, PNGase F-II, PNGase H+, PNGase J, PNGase Os, PNGase Se, PNGase Yl, PNGase-A, PNGaseF, Pngl, SpPNGase, TGc domain-containing protein, yeast peptide: N-glycanase, YPng, YPng1

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.52 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

Purification

Purification on EC 3.5.1.52 - peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
14500fold
Prunus amygdalus var. dulcis
-
460fold
-
glutathione-Sepharose column chromatography
native enzyme by Fe2+ affinity and phosphopeptide affinity chromatography to over 95% purity
-
Ni-nitrilotriacetate column chromatography
recombinant full-length enzyme, enzyme core domain, and enzyme XPCB domain from Escherichia coli strain BL21(DE3),the XPCB domain by chitin affinity chromatographyand gel filtration
-
recombinant GST-tagged proteasome components HR23B, S4, and AMFR from Escherichia coli by glutathione affinity batch method, recombinant His6-tagged wild-type enzyme and truncated variants from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography
-
recombinant His-tagged enzyme PNGase Yl from Pichia pastoris strain GS115 by ultracentrifugatio, dialysis, and nickel affinity chromatography
-
recombinant His6-tagged wild-type and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant wild-type and mutant enzyme fusion PUB domain from Escherichia coli strain C41(DE3) by nickel affinity chromatography, ion exchange chromatography, and gel filtration
-
recombinant wild-type and mutant Png1 from Escherichia coli strain BL21(DE3)
-