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3.5.1.52: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

This is an abbreviated version!
For detailed information about peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase, go to the full flat file.

Word Map on EC 3.5.1.52

Reaction

Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser
+
H2O
=
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc
 +
Asn-Asp-Glu-Ser-Ser

Synonyms

acid PNGase M, acidic peptide:N-glycanase, acidic PNGase, aPNGase, At3g14920, At5g05480, AtPNG1, DDB0189828, EC 3.2.2.18, glycoamidase, glycopeptidase, glycopeptide N-glycosidase, jackbean glycopeptidase, L-929 PNGase, MPng1, N-glycanase, N-glycosidase F, N-oligosaccharide glycopeptidase, Ncpng1, neutral PNGase M, Ngly1, peptide N-glycanase, peptide N-glycosidase, peptide N-glycosidase F, peptide N4(N-acetyl-glucosaminyl)asparagine amidase, peptide-N-(N-acetyl-beta-glucosaminyl) asparagine amidase F, peptide-N-glycanase, peptide-N-glycosidase F, peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase, peptide: N-glycanase, peptide: N-glycosidase, peptide:N-glycanase, peptide:N-glycanase homolog, peptide:N-glycosidase, peptidyl N-glycanase F, PGNase, PNG-1, PNG1, Png1p, PNGase, PNGase A, PNGase A-like enzyme, PNGase At, PNGase F, PNGase F-II, PNGase H+, PNGase J, PNGase Os, PNGase Se, PNGase Yl, PNGase-A, PNGaseF, Pngl, SpPNGase, TGc domain-containing protein, yeast peptide: N-glycanase, YPng, YPng1

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.52 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

Inhibitors

Inhibitors on EC 3.5.1.52 - peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
20% inhibition at 100 mM
benzyloxycarbonyl-VAD-fluoromethyl ketone
-
-
benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone
-
covalently bind to the active site Cys191 of recombinant yeast peptide N-glycanase
Ca2+
2 mM, 19% inhibition
carbobenzyloxy-Val-Ala-Asp
carbobenzyloxy-Val-Ala-Asp-alpha-fluoromethylketone
CuSO4
-
nearly complete inhibition of the acid PNGase M by 5 mM, 10-20% remaining activity at neutral PNGase M by 5 mM
Fbs1
-
Fbs1 captures malfolded glycoproteins, protecting them from the attack of PNGase, during the chaperoning or ubiquitinating operation in the cytosol
-
Fe2+
2 mM, 90% inhibition
FeCl3
-
nearly complete inhibition of the acid PNGase M by 5 mM, 10-20% remaining activity at neutral PNGase M by 5 mM
fucose residue alpha-1-3-linked or alpha-1-6-linked to the proximal GlnNAc residue
-
complete inhibition
-
Glycoasparagine
K+
-
significant inactivation by 100 mM
L-hyosphorin derived nonapeptide
-
1 mM causes 92% inbition of acid PNGase M
-
liberated glycan reaction product
-
Man8GlcNAc2-iodoacetoamide
-
-
Man9GlcNAc2-iodoacetoamide
-
strong inhibitor, irreversibly inhibits that catalytic Cys in a highly specific manner
Mg2+
2 mM, 14% inhibition
Monoiodoacetic acid
N,N'-diacetylchitobiose
N,N'-diacetylchitobiosylbromoacetamide
-
-
N,N'-diacetylchitobiosylchloroacetamide
-
-
N,N'-diacetylchitobiosyliodoacetamide
-
-
N-benzyloxycarbonyl-VAD-fluoromethylketone
Z-VAD-fmk
N-ethylmaleimide
Na+
-
significant inactivation by 100 mM
p-chloromercuribenzoic acid
-
0.5 mM causes 89% inhibition of neutral PNGase M
phosphate buffers
-
no significant activity
-
SDS
-
complete inactivation by 0.05% SDS can be corrected by the addition of 1% Nonidet P-40
triomannose
Tris-HCl
-
no significant activity
Triton X100
-
40% inhibition at 1%
Urea
-
80% inhibition at 2 M
yeast mannan
-
-
-
Z-VAD-fmk
ZnCl2
-
10-20% remaining activity at neutral PNGase M by 5 mM
additional information
-