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3.5.1.52: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

This is an abbreviated version!
For detailed information about peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase, go to the full flat file.

Word Map on EC 3.5.1.52

Reaction

Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser
+
H2O
=
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc
 +
Asn-Asp-Glu-Ser-Ser

Synonyms

acid PNGase M, acidic peptide:N-glycanase, acidic PNGase, aPNGase, At3g14920, At5g05480, AtPNG1, DDB0189828, EC 3.2.2.18, glycoamidase, glycopeptidase, glycopeptide N-glycosidase, jackbean glycopeptidase, L-929 PNGase, MPng1, N-glycanase, N-glycosidase F, N-oligosaccharide glycopeptidase, Ncpng1, neutral PNGase M, Ngly1, peptide N-glycanase, peptide N-glycosidase, peptide N-glycosidase F, peptide N4(N-acetyl-glucosaminyl)asparagine amidase, peptide-N-(N-acetyl-beta-glucosaminyl) asparagine amidase F, peptide-N-glycanase, peptide-N-glycosidase F, peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase, peptide: N-glycanase, peptide: N-glycosidase, peptide:N-glycanase, peptide:N-glycanase homolog, peptide:N-glycosidase, peptidyl N-glycanase F, PGNase, PNG-1, PNG1, Png1p, PNGase, PNGase A, PNGase A-like enzyme, PNGase At, PNGase F, PNGase F-II, PNGase H+, PNGase J, PNGase Os, PNGase Se, PNGase Yl, PNGase-A, PNGaseF, Pngl, SpPNGase, TGc domain-containing protein, yeast peptide: N-glycanase, YPng, YPng1

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.52 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

Cloned

Cloned on EC 3.5.1.52 - peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of His-tagged or GST-tagged truncated enzyme variants and of GST-tagged enzyme mutants with His-tagged p97, transient co-expression and complex formatin of N-terminal GFP-tagged PNGase and c-myc-tagged AMFR in COS-1 cells
-
co-expression of His6-tagged full-length enzyme or truncated versions with full-length Rad23 Escherichia coli strain BL21(DE3)
-
co-expression of the His6-tagged wild-type enzyme and truncated variants with GST-tagged murine proteasome components HR23B, S4, and autocrine motility factor receptor AMFR in Escherichia coli
-
DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) Codon-Plus cells
expressed in Escherichia coli strain JM109 and in Pichia pastoris strain GS115
expressed in Saccharomyces cerevisiae
-
expressed in Sf21 insect cells
-
expression in Escherichia coli
expression in Escherichia coli and CHO cells
expression in Escherichia coli as inclusion bodies
-
expression of HA-tagged wild-type and mutant enzymes in U373 astrocytoma cells
-
expression of His- or FLAG-tagged wild-type and mutant enzymes, co-expression with Rad23
-
expression of the enzyme in U373 astrocytoma cells
-
expression of wild-type and mutant enzyme PUB domain, comprising residues 11-109, fused to the lipoyl domain of Bacillus stearothermophilus dehihydrolipoamide acetyltransferase in Escherichia coli strain C41(DE3)
-
gene DDB0189828, sequence comparisons and phylogenetic analysis
gene Ddpngase, single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression under control of the constitutive promoter actin 15 and fused to enhanced yellow fluorescent protein (EYFP), in the mutant axenic strain Ax2
gene Ncpng1, sequence comparisons and phylogenetic analysis
gene NGLY1, sequence comparisons and phylogenetic analysis
gene png1, expression of wild-type and mutant Png1 in Escherichia coli strain BL21(DE3)
-
gene PNG1, sequence comparisons and phylogenetic analysis
gene PNGase F-II, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His6-tagged wild-type and selenomethionine-labeled enzyme in Escherichia coli strain BL21(DE3)
importantly, heterologous expression of AtPNG1 restores N-glycanase activity in a PNGase-deficient Saccharomyces cerevisiae mutant
-
into the vector pET28a for expression in Escherichia coli BL21DE3 Codon Plus RIL cells
overexpression of the full-length enzyme, enzyme core domain, and enzyme XPCB domain in Escherichia coli strain BL21(DE3)
-
PNGase Yl, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115
-
recombinant expression in an Arabidopsis thaliana aPNGase-knockout line
the PNGase F gene from Flavobacterium meningosepticum is cloned into plasmid pYD1, which enables regulated expression, secretion and detection. The expression of PNGase F gene at extracellular surface of Saccharomyces cerevisiae is confirmed by immunofluorescence microscopy. Fluorescence activated cell sorter analysis indicates that, after 36 h cultivation, 47.6% of the cell surface is anchored with target proteins. The surface engineered enzyme is confirmed to be active and reached its highest level after induced for 36 h
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