3.5.1.42: nicotinamide-nucleotide amidase

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For detailed information about nicotinamide-nucleotide amidase, go to the full flat file.

Word Map on EC 3.5.1.42

3.5.1.42

pyridine

nicotinic

nad+

salvage

nmnat2

typhimurium

phosphoribosyltransferase

glycohydrolase

preiss-handler

adp-ribose

amidohydrolases

four-membered

dyad

pyrophosphatases

riboside

deamidates

injured

Reaction

Synonyms

AtCinA, CinA, nicotinamide mononucleotide amidohydrolase, nicotinamide mononucleotide deamidase, nicotinamide mononucleotide deaminase, nicotinamide nucleoside amidase, Nm deamidase, NMN deamidase, NMN deaminase, OiPncC, PncC, TK1650, YgaD

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.42 nicotinamide-nucleotide amidase

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Results	in table
3	Activating Compound
2395	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
2	Disease/ Diagnostics
21	General Information
2	General Stability
15	Inhibitors
10	kcat/KM [mM/s]
7	Ki Value [mM]
20	KM Value [mM]
1	Localization
2	Metals/Ions
9	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
36	Organism
5	Pathway
8	pH Optimum
4	pH Range
5	pH Stability
29	Protein Variants
10	Purification (Commentary)
5	Reaction
1	Reaction Type
36	Reference
20	Source Tissue
6	Specific Activity [micromol/min/mg]
3	Storage Stability
53	Substrates and Products (Substrate)
12	Subunits
41	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
12	Turnover Number [1/s]

Purification

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Purification on EC 3.5.1.42 - nicotinamide-nucleotide amidase

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Aspergillus terreus

663668

ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Reactive Red 120-agarose column chromatography, Resource Q column chromatography, and Superose 12 gel filtration

Shewanella oneidensis

719984

near homogeneity, chromatography techniques

Escherichia coli

209136

partial

2 entries

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration

Oceanobacillus iheyensis

Q8EQR8

735110

recombinant His6-tagged enzyme from Escherichia coli strain T7 by nickel affinity chromatgraphy, cleavage of the tag by thrombin, anion exchange chromatography, and gel filtration

Thermus thermophilus

Q5SHB0

734285

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nicke affinity chromatography

Escherichia coli

733870

to homogeneity, chromatography techniques

Azotobacter vinelandii

209140