3.5.1.42: nicotinamide-nucleotide amidase

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For detailed information about nicotinamide-nucleotide amidase, go to the full flat file.

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Synonyms

AtCinA, CinA, nicotinamide mononucleotide amidohydrolase, nicotinamide mononucleotide deamidase, nicotinamide mononucleotide deaminase, nicotinamide nucleoside amidase, Nm deamidase, NMN deamidase, NMN deaminase, OiPncC, PncC, TK1650, YgaD

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.42 nicotinamide-nucleotide amidase

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Results	in table
3	Activating Compound
2395	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
2	Disease/ Diagnostics
21	General Information
2	General Stability
15	Inhibitors
10	kcat/KM [mM/s]
7	Ki Value [mM]
20	KM Value [mM]
1	Localization
2	Metals/Ions
9	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
36	Organism
5	Pathway
8	pH Optimum
4	pH Range
5	pH Stability
29	Protein Variants
10	Purification (Commentary)
5	Reaction
1	Reaction Type
36	Reference
20	Source Tissue
6	Specific Activity [micromol/min/mg]
3	Storage Stability
53	Substrates and Products (Substrate)
12	Subunits
41	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
12	Turnover Number [1/s]

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Cloned on EC 3.5.1.42 - nicotinamide-nucleotide amidase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

gene cinA, recombinant expression of His6-tagged enzyme with a thrombin cleavage site at the N terminus in Escherichia coli strain T7

Thermus thermophilus

Q5SHB0

734285

gene cinA, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Rosetta 2(DE3)

Agrobacterium fabrum

A9CJ26

755141

gene pncC, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3)

Oceanobacillus iheyensis

Q8EQR8

735110

gene pncC, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Escherichia coli

-

733870

gene TK1650, recombinant expression in Thermococcus kodakarensis strain KU216

Thermococcus kodakarensis

Q5JIT6

754020

recombinant expression of NMN deamidase fused to EGFP in transgenic mice and zebrafish larvae

Escherichia coli

-

753342