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3.5.1.38: glutamin-(asparagin-)ase

This is an abbreviated version!
For detailed information about glutamin-(asparagin-)ase, go to the full flat file.

Word Map on EC 3.5.1.38

Reaction

L-glutamine
+
H2O
=
L-glutamate
+
NH3

Synonyms

asparaginase A, asparaginase B, glutaminase-asparaginase, glutaminase/asparaginase, periplasmic glutaminase/asparaginase, PGA

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.38 glutamin-(asparagin-)ase

Storage Stability

Storage Stability on EC 3.5.1.38 - glutamin-(asparagin-)ase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, lyophilized to dryness in the presence of 50 mM Tris, 250 mM glycine, pH 9.0, 90% activity in 3 months
-
4°C, 12 months, asparaginase A, asparaginase B: DTT required, 6 months
-
4°C, 50 mM borate buffer, pH 7.0, 100 mM NaCl, 1 mM EDTA, 3 to 4 months
-
activity is retained in the freezer for some months
-