3.5.1.36: N-methyl-2-oxoglutaramate hydrolase

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Reaction

Synonyms

5-hydroxy-N-methylpyroglutamate synthase, 5-hydroxy-N-methylpyroglutamate synthetase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.36 N-methyl-2-oxoglutaramate hydrolase

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Results	in table
5	Activating Compound
1	CAS Registry Number
14	Inhibitors
6	KM Value [mM]
1	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
36	Organism
1	pH Optimum
1	Purification (Commentary)
1	Reaction
3	Reaction Type
8	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
10	Substrates and Products (Substrate)
2	Synonyms
1	Systematic Name
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.5.1.36 - N-methyl-2-oxoglutaramate hydrolase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

extraction, addition of streptomycin sulfate, adjust the pH value, centrifugation, ammonium sulfate precipitation, dialysis, purification with DEAE, Sephadex and hydroxyapatite column with dialysis and ultrafiltration steps

Pseudomonas sp.

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209087