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3.5.1.33: N-acetylglucosamine deacetylase

This is an abbreviated version!
For detailed information about N-acetylglucosamine deacetylase, go to the full flat file.

Word Map on EC 3.5.1.33

Reaction

N-acetyl-D-glucosamine
+
H2O
=
D-glucosamine
+
acetate

Synonyms

acetylaminodeoxyglucose acetylhydrolase, Dacph, glucosaminyl N-deacetylase, Lmo0415, N-acetyl-D-glucosaminyl N-deacetylase, ND, Pgd, pgdA, Ph-Dac, PH0499, Tk-Dac, TK1764

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.33 N-acetylglucosamine deacetylase

Cloned

Cloned on EC 3.5.1.33 - N-acetylglucosamine deacetylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression analysis through RT-PCR
-
expression in Escherichia coli
expression of a combined glucosaminyl N-deacetylase/N-sulfotransferase in human kidney cell line 293
-
overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys. The insoluble inclusion body is solubilized and reactivated through a refolding procedure