3.5.1.19: nicotinamidase
This is an abbreviated version!
For detailed information about nicotinamidase, go to the full flat file.
Word Map on EC 3.5.1.19
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3.5.1.19
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mycobacterium
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tuberculosis
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pyrazinamide
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pyrazinoic
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pza-resistant
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bactec
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pyrazinamide-resistant
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sir2-dependent
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kansasii
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wayne
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sir2-mediated
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pza-susceptible
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analysis
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drug development
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medicine
- 3.5.1.19
- mycobacterium
- tuberculosis
- pyrazinamide
-
pyrazinoic
-
pza-resistant
-
bactec
-
pyrazinamide-resistant
-
sir2-dependent
- kansasii
-
wayne
-
sir2-mediated
-
pza-susceptible
- analysis
- drug development
- medicine
Reaction
Synonyms
AS87_01735, ASAC_0847, NAAA, NA_As0847, NIC, nicotinamidase, nicotinamidase PNC-1, nicotinamidase Pnc1p, nicotinamidase PncA, nicotinamidase/pyrazinamidase, nicotinamidase/pyrazinamidase PncA, nicotinamide amidase, nicotinamide deaminase, Nicotine deamidase, OiNIC, PNC-1, Pnc1, Pnc1p, PNC2, PncA, polygenomic nicotinamidase, PolyNic, pyrazinamidase, PZAase, PZAse, SpNic, TTHA0328, UbNic, YNDase
ECTree
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Turnover Number
Turnover Number on EC 3.5.1.19 - nicotinamidase
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0.0088
Benzamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
2.16
recombinant mutant Q96K, pH 7.3, 37°C
5.1
5-methyl nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
7.09
5-methyl nicotinamide
recombinant mutant C133A, pH 7.3, 37°C
15.9
5-methyl nicotinamide
recombinant mutant Q96A, pH 7.3, 37°C
1.75
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
4.9
5-Methylnicotinamide
37°C, pH 7.3, mutant enzyme H75E
9.6
5-Methylnicotinamide
37°C, pH 7.3, mutant enzyme A151C
12.4
5-Methylnicotinamide
37°C, pH 7.3, mutant enzyme H75S
13.7
5-Methylnicotinamide
37°C, pH 7.3, mutant enzyme W83F
43.7
5-Methylnicotinamide
37°C, pH 7.3, wild-type enzyme
0.1
ethyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
0.1
37°C, pH 7.3, mutant enzyme H75E
0.2
ethylnicotinate
37°C, pH 7.3, mutant enzyme H75S
0.2
ethylnicotinate
37°C, pH 7.3, mutant enzyme W83F
0.3
ethylnicotinate
37°C, pH 7.3, mutant enzyme A151C
0.32
methyl nicotinate
recombinant wild-type enzyme, pH 7.3, 37°C
1.02
methyl nicotinate
recombinant mutant C133A, pH 7.3, 37°C
1.9
37°C, pH 7.3, mutant enzyme H75S
4.3
methylnicotinate
37°C, pH 7.3, mutant enzyme A151C
4.9
methylnicotinate
37°C, pH 7.3, mutant enzyme W83F
5.8
methylnicotinate
37°C, pH 7.3, mutant enzyme H75E
54.5
methylnicotinate
37°C, pH 7.3, wild-type enzyme
0.0005
kcat below 0.0005 s-1, mutant enzyme C167A, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.0006
nicotinamide
mutant enzyme D8E, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.0009
nicotinamide
mutant enzyme D8A, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.0009
nicotinamide
mutant enzyme D8N, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.0009
nicotinamide
mutant enzyme K122R, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.003
nicotinamide
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wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
0.007
nicotinamide
mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.014
nicotinamide
mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.02
nicotinamide
mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.044
nicotinamide
mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.064
nicotinamide
mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C
0.28
nicotinamide
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wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
0.3
nicotinamide
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wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
0.5
nicotinamide
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mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C
0.69
nicotinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
2.1
nicotinamide
-
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
3.1
nicotinamide
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wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
3.1
nicotinamide
-
wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C
3.8
nicotinamide
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wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
9.82
nicotinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C
11.65
nicotinamide
recombinant wild-type enzyme, pH 7.3, 37°C
50
nicotinamide
pH and temperature not specified in the publication
76.9
nicotinamide
pH and temperature not specified in the publication
0.1
-
mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C
2.56
Pyrazinamide
wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C
3.8
Pyrazinamide
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wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C
5.21
Pyrazinamide
recombinant mutant F68W/C133A, pH 7.3, 37°C