Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.19: nicotinamidase

This is an abbreviated version!
For detailed information about nicotinamidase, go to the full flat file.

Word Map on EC 3.5.1.19

Reaction

nicotinamide
+
H2O
=
nicotinate
 +
NH3

Synonyms

AS87_01735, ASAC_0847, NAAA, NA_As0847, NIC, nicotinamidase, nicotinamidase PNC-1, nicotinamidase Pnc1p, nicotinamidase PncA, nicotinamidase/pyrazinamidase, nicotinamidase/pyrazinamidase PncA, nicotinamide amidase, nicotinamide deaminase, Nicotine deamidase, OiNIC, PNC-1, Pnc1, Pnc1p, PNC2, PncA, polygenomic nicotinamidase, PolyNic, pyrazinamidase, PZAase, PZAse, SpNic, TTHA0328, UbNic, YNDase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.19 nicotinamidase

Temperature Stability

Temperature Stability on EC 3.5.1.19 - nicotinamidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 20
purified recombinant wild-type enzyme, 20 h, stable
40
-
pH 6.8, in presence of Co2+, 10 min, stable up to
55
-
half-life: 2.5 min
65
-
half-life: 1 min
70
-
10 min, complete loss of activity
71.3
melting temperature in glycine (pH 10.0)
84.7
melting temperature in glycine buffer pH 9.0
84.8
melting temperature in sodium phosphate buffer pH 7.3
additional information
ammonium sulfate at 1 M is able to produce a slight increase in its Tm (86.4°C) in comparison with that found in sodium phosphate pH 7.3. The nicotinamidase reaction products, nicotinic and pyrazinoic acids improved UbNic stability, increasing its Tm to 87.7°C and 87.1°C. Nicotinaldehyde and 5-bromonicotinaldehyde, increased Tm up to 92.7°C and 85.7°C