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3.5.1.19: nicotinamidase

This is an abbreviated version!
For detailed information about nicotinamidase, go to the full flat file.

Word Map on EC 3.5.1.19

Reaction

nicotinamide
+
H2O
=
nicotinate
 +
NH3

Synonyms

AS87_01735, ASAC_0847, NAAA, NA_As0847, NIC, nicotinamidase, nicotinamidase PNC-1, nicotinamidase Pnc1p, nicotinamidase PncA, nicotinamidase/pyrazinamidase, nicotinamidase/pyrazinamidase PncA, nicotinamide amidase, nicotinamide deaminase, Nicotine deamidase, OiNIC, PNC-1, Pnc1, Pnc1p, PNC2, PncA, polygenomic nicotinamidase, PolyNic, pyrazinamidase, PZAase, PZAse, SpNic, TTHA0328, UbNic, YNDase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.19 nicotinamidase

Purification

Purification on EC 3.5.1.19 - nicotinamidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilized metal affinity chromatography
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Sephacryl S-300 gel filtration
Ni-NTA resin column chromatography
nickel chelate chromatography and molecular sieve
-
nickel-based affinity chromatography
-
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes methylnicotinamide from Escherichia coli strain Rosetta 2 (DE3) by ultrafiltration and nickel affinity chromatography