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3.5.1.19: nicotinamidase

This is an abbreviated version!
For detailed information about nicotinamidase, go to the full flat file.

Word Map on EC 3.5.1.19

Reaction

nicotinamide
+
H2O
=
nicotinate
+
NH3

Synonyms

AS87_01735, ASAC_0847, NAAA, NA_As0847, NIC, nicotinamidase, nicotinamidase PNC-1, nicotinamidase Pnc1p, nicotinamidase PncA, nicotinamidase/pyrazinamidase, nicotinamidase/pyrazinamidase PncA, nicotinamide amidase, nicotinamide deaminase, Nicotine deamidase, OiNIC, PNC-1, Pnc1, Pnc1p, PNC2, PncA, polygenomic nicotinamidase, PolyNic, pyrazinamidase, PZAase, PZAse, SpNic, TTHA0328, UbNic, YNDase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.19 nicotinamidase

Crystallization

Crystallization on EC 3.5.1.19 - nicotinamidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure (PDB ID 2WT9) analysis, and modeling of substrate binding and unbinding, overview
sitting-drop vapor-diffusion method at 14°C. The crystal structure is determined at 2.0 A resolution. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers are observed in crystals, but only dimers are observed in solution
sitting-drop-based and sparse-matrix screening , using 0.7 M Na-citrate and 0.1 M HEPES (pH 7.5)
-
in complex with nicotinaldehyde, hanging drop vapor diffusion method, using 1.6 M NaOAc, 10% (w/v) ethylene glycol, and 0.1 M HEPES (pH 7.4)
resolution 2.9 Angstrom
a trapped nicotinoyl-thioester complexed with wild type enzyme and C136S mutant in complex with nicotinamide, hanging drop vapor diffusion method, using 18-22% (w/v) polyethylene glycol 3350, 0.2-0.3 M NaCl and 0.2 M sodium malonate, pH 6.3, at 18°C
crystal structure (PDB ID 3O94) analysis, and modeling of substrate binding and unbinding, overview