3.5.1.18: succinyl-diaminopimelate desuccinylase
This is an abbreviated version!
For detailed information about succinyl-diaminopimelate desuccinylase, go to the full flat file.
Word Map on EC 3.5.1.18
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3.5.1.18
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haemophilus
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l-captopril
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peptidoglycan
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meso-diaminopimelic
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acetylornithine
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metallohydrolase
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drug development
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medicine
- 3.5.1.18
- haemophilus
- l-captopril
- peptidoglycan
-
meso-diaminopimelic
- acetylornithine
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metallohydrolase
- drug development
- medicine
Reaction
Synonyms
Cgl1109, DapE, dapE-encoded N-succinyl-LL-diaminopimelic acid desuccinylase, HiDapE, N-succinyl-L,L-diaminopimelic acid desuccinylase, N-succinyl-L-alpha,epsilon-diaminopimelic acid deacylase, Rv1202, S-DAP deacylase, SDAP, sDap desuccinylase, succinyl-diaminopimelate desuccinylase, succinyldiaminopimelate desuccinylase
ECTree
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Reaction
Reaction on EC 3.5.1.18 - succinyl-diaminopimelate desuccinylase
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N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate
structure-activity relationship and catalytic mechanism of peptide bond cleavage by DapE enzymes, overview. The catalytic domain is composed of residues 1-179 and 293-376
N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate
the catalytic reaction progresses via a general acid-base hydrolysis mechanism where Glu134 first acts as a Lewis base by activating the catalytic water molecule in the active site, followed by guiding the resulting hydroxyl ion for a nucleophilic attack on the substrate, and finally acts as a Lewis acid by donating a proton to the substrate. Catalytic mechanism and intermediates and transition states, hybrid QM/MM computational method analysis, overview. A conformational change in the side chain of Asp100, which bridges the two Zn centers of the enzyme, is observed which facilitates the enzymatic action by lowering the activation energy and leads to the formation of a different intermediate during the catalytic reaction. The nucleophilic attack is the rate determining step
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