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3.5.1.18: succinyl-diaminopimelate desuccinylase

This is an abbreviated version!
For detailed information about succinyl-diaminopimelate desuccinylase, go to the full flat file.

Word Map on EC 3.5.1.18

Reaction

N-succinyl-LL-2,6-diaminoheptanedioate
+
H2O
=
succinate
 +
LL-2,6-Diaminoheptanedioate

Synonyms

Cgl1109, DapE, dapE-encoded N-succinyl-LL-diaminopimelic acid desuccinylase, HiDapE, N-succinyl-L,L-diaminopimelic acid desuccinylase, N-succinyl-L-alpha,epsilon-diaminopimelic acid deacylase, Rv1202, S-DAP deacylase, SDAP, sDap desuccinylase, succinyl-diaminopimelate desuccinylase, succinyldiaminopimelate desuccinylase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.18 succinyl-diaminopimelate desuccinylase

Crystallization

Crystallization on EC 3.5.1.18 - succinyl-diaminopimelate desuccinylase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting nanodroplet vapor diffusion method, using 6 mM ZnCl2, 43.1% (w/v) polyethylene glycol 400, 0.2 M sodium chloride, 0.1 M sodium/potassium phosphate pH 6.4, at 20°C
crystal structure of the enzyme in complex with the products succinic acid and diaminopimelic acid, crystal structure is determined at 1.95 A
crystal structure, PDB ID 3IC1, analysis, comparison with the structure of N-acetyl-L-ornithine deacetylase, EC 3.5.1.16, overview
-
purified recombinant wild-type and mutant G172D enzymes, apoform and Zn2+-bound enzyme, using 400 nl of a precipitant solution containing 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG 3350, and 400 nl of 15 mg/ml of protein in crystallization buffer, with or without 1 mM ZnCl2, within 14 days, X-ray diffraction structure determination and analysis at 1.84 A resolution
-
ultrapure recombinant DapE with one and two zinc ions bound in the active site, respectively, at 16°C, by vapor diffusion in hanging drops containing 1 ml of precipitant solution containing 1 M ammonium sulfate, 0.2 M NaCl, and 0.1 M Na acetate, pH 4.4, and 0.001 ml of 13 mg/ml of DapE with three equivalents of zinc, 2 weeks, X-ray diffraction structure determination and analysis at 2.0-2.3 A resolution
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001-0.002 ml of 3-7 mg/ml protein in 50 mM Bis-Tris, pH 6.0, 200 mM NaCl, 0.5 mM TCEP is mixed with 0.001-0.002 ml reservoir solution, containing 5-10% w/v PEG 4000 or PEG 3350, 35-120 mM ammonium sulfate, 100 mM sodium acetate, pH 4.1-4.6, and equilibrated against 0.9 ml reservoir solution, at room temperature, 1 day, method optimization, X-ray diffraction structure determination and analysis at 2.4-2.58 A resolution, two crystal forms
sitting-drop vapor-diffusion method at 16°C, three-dimensional X-ray crystal structure of the enzyme in complex with L-captopril at 1.8 A resolution
purified recombinant enzyme, apoform and Zn2+-bound enzyme, using 400 nl of a precipitant solution containing 20% v/v 1,4-butanediol, 0.1 M sodium acetate, pH 4.5, and 400 nl of 19 mg/ml protein in crystallization buffer, with or without 1 mM ZnCl2, within 14 days, X-ray diffraction structure determination and analysis at 1.65 A resolution