Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.124: protein deglycase

This is an abbreviated version!
For detailed information about protein deglycase, go to the full flat file.

Word Map on EC 3.5.1.124

Reaction

an N6-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine
+
H2O
=
a [protein]-L-lysine
 +
Lactate

Synonyms

deglycase, DJ-1, DJ-1 deglycase, DJ-1 protein, glutathione-independent glyoxalase, glyoxalase, glyoxylase III, hchA, Hsp31, Maillard deglycase, More, PARK7, Parkinsonism-associated protein, Parkinsonism-associated protein DJ-1, PfpI, yajL, yhbO

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.124 protein deglycase

Cloned

Cloned on EC 3.5.1.124 - protein deglycase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in pET28a+ vector, overexpressed in Escherichia coli BL21 (DE3) cells as N-terminus 6×His tagged fusion protein
DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells
recombinant expression in Escherichia coli from pET21a-DJ-1 plasmid