3.5.1.122: protein N-terminal glutamine amidohydrolase

This is an abbreviated version!
For detailed information about protein N-terminal glutamine amidohydrolase, go to the full flat file.

Reaction

Synonyms

C8orf32, Cg8253, CT1, FLJ10204, Gln-specific amino-terminal (Nt)-amidase, glutamine-specific N-terminal amidase, hNtaq1, human N-terminal glutamine amidohydrolase isoform 1, N-terminal amidase, Nt-amidase, NTA1, Ntaq, NTAQ1, NtQ-amidase, Wdyhv1, yNta1

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.122 protein N-terminal glutamine amidohydrolase

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Results	in table
2079	AA Sequence
5	Cloned(Commentary)
2	Crystallization (Commentary)
1	Expression
19	General Information
1	KM Value [mM]
2	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
8	Organism
1	Pathway
2	PDB ID
1	pH Optimum
2	Purification (Commentary)
2	Reaction
8	Reference
2	Source Tissue
11	Substrates and Products (Substrate)
4	Subunits
29	Synonyms
1	Systematic Name

Substrates Products

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Substrates Products on EC 3.5.1.122 - protein N-terminal glutamine amidohydrolase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

N-terminal L-glutaminyl-[DHFRbt] + H2O

N-terminal L-glutamyl-[DHFRbt] + NH3

Mus musculus

Q80WB5

substrate is Escherichia coli dihydrofolate reductase (DHFR) moiety fused to a C-terminal peptide (denoted as bt) that is biotinylated in vivo

741003

-

-

?

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

6 entries

QKGSGAW + H2O

EKGSGAW + NH3

Arabidopsis thaliana

O22944

-

754918

-

-

?

QLGSGAW + H2O

ELGSGAW + NH3

Arabidopsis thaliana

O22944

about 80% of the activity compared to QKGSGAW

754918

-

-

?

additional information

?

-

3 entries

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

Bos taurus

Q3T0D3

-

741003

-

-

?

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

Drosophila melanogaster

Q7K2Y9

-

741003

-

-

?

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

Homo sapiens

Q96HA8

-

741003, 741295

-

-

?

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

Homo sapiens

-

-

741295

-

-

?

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

Mus musculus

Q80WB5

-

741003

-

-

?

N-terminal L-glutaminyl-[protein] + H2O

N-terminal L-glutamyl-[protein] + NH3

Saccharomyces cerevisiae

P40354

-

741343

-

-

?

additional information

?

-

Escherichia coli

-

the amidohydrolase activity of the native enzyme and of proteolytic fragments is analyzed using either L-glutamine or gamma-L-glutamic 4-nitroanilide as substrates

739951

-

-

?

additional information

?

-

Saccharomyces cerevisiae

P40354

dual specificity of yeast Nta1 (yNta1), importance of second-position residues in Asn/Gln-bearing N-terminal degradation signals (N-degrons), also cf. EC 3.5.1.121. Specific hydrogen bonds stabilize interactions between N-degron peptides and hydrophobic peripheral regions of the active site pocket, interactions between Nta1 and N-degron peptides, detailed overview. The enzyme shows glutamine-specific enzyme activity with dipeptides Gln-Val and Gln-Gly, Michaelis-Menten kinetics

741343

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

dual specificity of yeast Nta1 (yNta1), importance of second-position residues in Asn/Gln-bearing N-terminal degradation signals (N-degrons), also cf. EC 3.5.1.121. Specific hydrogen bonds stabilize interactions between N-degron peptides and hydrophobic peripheral regions of the active site pocket, interactions between Nta1 and N-degron peptides, detailed overview. The enzyme shows glutamine-specific enzyme activity with dipeptides Gln-Val and Gln-Gly, Michaelis-Menten kinetics

741343

-

-

?