3.5.1.122: protein N-terminal glutamine amidohydrolase
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For detailed information about protein N-terminal glutamine amidohydrolase, go to the full flat file.
Reaction
Synonyms
C8orf32, Cg8253, CT1, FLJ10204, Gln-specific amino-terminal (Nt)-amidase, glutamine-specific N-terminal amidase, hNtaq1, human N-terminal glutamine amidohydrolase isoform 1, N-terminal amidase, Nt-amidase, NTA1, Ntaq, NTAQ1, NtQ-amidase, Wdyhv1, yNta1
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Purification
Purification on EC 3.5.1.122 - protein N-terminal glutamine amidohydrolase
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native enzyme from a brain extract by ion exchange, hydrophobic chromatography, and gel filtration
recombinant N-terminally His6-MBP-tagged enzyme from Escherichia coli strain B834 as Se-Met labeled protein by nickel affinity chromatography, tag cleavage by TEV protease, gel filtration,and another step of nickel affinity chromatography, followed by desalting gel filtration