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3.5.1.121: protein N-terminal asparagine amidohydrolase

This is an abbreviated version!
For detailed information about protein N-terminal asparagine amidohydrolase, go to the full flat file.

Word Map on EC 3.5.1.121

Reaction

N-terminal L-asparaginyl-[protein]
+
H2O
=
N-terminal L-aspartyl-[protein]
+
NH3

Synonyms

amidohydrolase for N-terminal asparagine, N-terminal amidase, N-terminal asparagine amidohydrolase, Nt-amidase, NTA1, NTAN1, NTAN1 amidase, NTAN1p amidase, NtN-amidase, PNAD, protein NH2-terminal asparagine deamidase, yNta1

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.121 protein N-terminal asparagine amidohydrolase

Purification

Purification on EC 3.5.1.121 - protein N-terminal asparagine amidohydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 11515fold to homogeneity from liver by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and another step of anion exchange chromatgraphy, follwed by chromatofocusing and again gel filtration
-
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity cromatography and ultrafiltration, method improvement