3.5.1.115: mycothiol S-conjugate amidase

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For detailed information about mycothiol S-conjugate amidase, go to the full flat file.

MCA, mycothiol S-conjugate amidase, Rv1082

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Results	in table
4443	AA Sequence
2	Application
5	Cloned(Commentary)
2	Expression
13	General Information
39	IC50 Value
75	Inhibitors
8	kcat/KM [mM/s]
22	KM Value [mM]
7	Metals/Ions
4	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
226	Organism
2	Pathway
1	pH Optimum
1	pH Stability
17	Protein Variants
5	Purification (Commentary)
1	Reaction
18	Reference
4	Specific Activity [micromol/min/mg]
45	Substrates and Products (Substrate)
7	Subunits
23	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
21	Turnover Number [1/s]

Specific Activity on EC 3.5.1.115 - mycothiol S-conjugate amidase

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0.0012

crude extract, at pH 7.5 and 30°C

0.13

crude extract, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C

10.6

after 82fold purification, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C

3.3

after 2800fold purification, at pH 7.5 and 30°C