3.5.1.114: N-acyl-aromatic-L-amino acid amidohydrolase
This is an abbreviated version!
For detailed information about N-acyl-aromatic-L-amino acid amidohydrolase, go to the full flat file.
Word Map on EC 3.5.1.114
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Synonyms
AA3, AAIII, Acy3, aminoacylase 3, aminoacylase III
ECTree
3.5.1.114 N-acyl-aromatic-L-amino acid amidohydrolaseAdvanced search results
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results (Metals Ions
Metals Ions on EC 3.5.1.114 - N-acyl-aromatic-L-amino acid amidohydrolase
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METALS and IONS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Co2+
Ni2+
Co2+ and to a lesser extent Ni2+ increases activity several times in comparison with intact wild type AA3. Co2+ drastically increases the rate of deacetylation of N-acetyl-1,2-dichlorovinyl-L-cysteine and significantly increased the toxicity of Ac-DCVC in the HEK293T cells expressing wt-AA3. Aminoacylase 3 is a metalloenzyme significantly activated by Co2+ and Ni2+
Zn2+
Wild type mouse aminoacylase 3 expressed in Escherichia coli contains 0.35 zinc atoms per monomer
additional information
Co2+
Co2+ and to a lesser extent Ni2+ increases activity several times in comparison with intact wild type AA3. Co2+ drastically increases the rate of deacetylation of N-acetyl-1,2-dichlorovinyl-L-cysteine and significantly increased the toxicity of Ac-DCVC in the HEK293T cells expressing wt-AA3. Aminoacylase 3 is a metalloenzyme significantly activated by Co2+ and Ni2+. The Co2+ -activated enzyme contains about 1 cobalt atom per monomer and trace amounts of zinc and other metals indicating that cobalt completely replaces them from the enzyme
additional information
Mn2+, Fe2+, Cd2+, Mg2+, Ca2+, K+ and Na+ do not significantly alter activity
