3.5.1.11: penicillin amidase
This is an abbreviated version!
For detailed information about penicillin amidase, go to the full flat file.
Word Map on EC 3.5.1.11
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3.5.1.11
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6-aminopenicillanic
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phenylacetic
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biocatalyst
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anandamide
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acylases
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cannabinoids
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cephalosporin
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synthesis
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binuclear
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alcaligenes
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cephalexin
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megaterium
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endocannabinoids
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semi-synthetic
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3.5.1.1
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kluyvera
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l-asparagine
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dihydroorotase
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phenylacetylated
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allantoinase
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dihydropyrimidinase
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lactonase
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amoxicillin
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rettgeri
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eupergit
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multipoint
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acyl-enzyme
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asparaginase
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hydantoinase
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phenoxyacetic
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aminoacylase
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7-aminocephalosporanic
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phosphotriesterase
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sphaericus
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hydantoin
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providencia
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n-acylethanolamines
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penicillanic
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dihydrouracil
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cleas
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pharmacology
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industry
- 3.5.1.11
-
6-aminopenicillanic
-
phenylacetic
-
biocatalyst
- anandamide
- acylases
- cannabinoids
- cephalosporin
- synthesis
-
binuclear
- alcaligenes
- cephalexin
- megaterium
-
endocannabinoids
-
semi-synthetic
-
3.5.1.1
-
kluyvera
- l-asparagine
- dihydroorotase
-
phenylacetylated
- allantoinase
- dihydropyrimidinase
- lactonase
- amoxicillin
- rettgeri
-
eupergit
-
multipoint
- acyl-enzyme
- asparaginase
- hydantoinase
-
phenoxyacetic
-
aminoacylase
-
7-aminocephalosporanic
- phosphotriesterase
- sphaericus
- hydantoin
- providencia
- n-acylethanolamines
-
penicillanic
- dihydrouracil
-
cleas
- pharmacology
- industry
Reaction
Synonyms
ACPGA001 PGA, AfPGA, alpha-acylamino-beta-lactam acylhydrolase, amidase, amidohydrolase, ampicillin acylase, AuAAC, benzylpenicillin acylase, BmPGA, Eca3205, KcPGA, maPGA, More, novozym 217, PA, PAC, penicillin acylase, penicillin amidase, Penicillin amidohydrolase, penicillin G acylase, Penicillin G amidase, Penicillin G amidohydrolase, penicillin V acylase, Penicillin V amidase, penicillin-G acylase, PGA, PGA650, PVA, semacylase, Sm-PVA, YxeI
ECTree
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Temperature Stability
Temperature Stability on EC 3.5.1.11 - penicillin amidase
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-20
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pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours
15 - 95
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temperature-induced unfolding studies of recombinant wild-type and mutant enzymes, kinetics, overview
24
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pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours
30
37
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pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours
40
45
50
55
60
70
82
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aculeacin A acylase is highly thermostable, with a midpoint transition temperature of 81.5°C
additional information
30
pH 6.0, 120 min, less than 5% loss of activity, wild-type enzyme and mutant enzyme T63S/S110C/N198Y
30
15 min, the enzyme retains 87% of its original activity
40
after 4 h at 40°C 80% residual activity, after 4 h at 50°C 40% residual activity, after 4 h at 55°C 20% residual activity, at 60°C rapidly inactivated
40
pH 6.0, 120 min, 5% loss of activity, wild-type enzyme and mutant enzyme T63S/S110C/N198Y
40
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fully stable at temperatures up to 40°C upon 1 h incubation at 8.5°C
40
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pH 8.0, half-life of the soluble enzyme is 397 h, and for the immobilized enzyme 625 h
40
purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 90% activity remaining
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pH 8.4, protein concentration of 1 mg/ml, 70% of activity lost within 8 hours
5
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pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours
50
purified recombinant enzyme, pH 6.6, 15 min, completely stable up to
50
pH 6.0, 120 min, about 75% loss of activity, wild-type enzyme. pH 6.0, 120 min, about 85% loss of activity, mutant enzyme T63S/S110C/N198Y
50
purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 50% activity remaining
55
purified recombinant enzyme, pH 6.6, 15 min, 80% activity remaining
55
purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 40% activity remaining, t1/2 is 60 min
60
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enzyme immobilized on epoxy polymer: 80% loss of activity after 30 min. Enzyme immobilized on amino polymers with short spacer arms: about 80% loss of activity after 60 min. Enzyme immobilized on amino polymers with long spacer arm: about 95% loss of activity after 5 min, about 80% loss of activity after 120 min. Native enzyme in solution: complete loss of activity after 5 min
60
pH 6.0, 120 min, about 75% loss of activity, wild-type enzyme. pH 6.0, 120 min, about 95% loss of activity, mutant enzyme T63S/S110C/N198Y
60
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pH 8.4, protein concentration of 1 mg/ml, complete denaturation within 8 hours
60
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enzyme immobilized on epoxy polymer: 90% loss of activity after 10 min. Enzyme immobilized on amino polymers with short spacer arms: about 65% loss of activity after 60 min, about 80% loss of activity after 180 min. Enzyme immobilized on amino polymers with long spacer arm: about 60% loss of activity after 60 min, about 80% loss of activity after 120 min. Native enzyme in solution: about 50% loss of activity after 45 min, about 90% loss of activity after 75 min
60
purified recombinant His-tagged enzyme, pH 7.4, 15 min, inactivation
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thermal stability of the enzyme immobilized on alginate/chitosan hybrid beads is much higher that that of the soluble enzyme
additional information
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study on inactivation kinetics over a wide pH-range at 25°C and 50°C. At temperatures above 35°C, enzyme undergoes a conformational change accompanied by destruction of salt bridges that maintain the active conformation