Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.108: UDP-3-O-acyl-N-acetylglucosamine deacetylase

This is an abbreviated version!
For detailed information about UDP-3-O-acyl-N-acetylglucosamine deacetylase, go to the full flat file.

Word Map on EC 3.5.1.108

Reaction

a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine
+
H2O
=
a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine
+
acetate

Synonyms

deacetylase, deacetylase LpxC, DHTase, EnvA protein, LpxC, LpxC deacetylase, LpxC enzyme, LpxC protein, UDP-(3-O-(R-3-hydroxymyristoyl))-N-acetylglucosamine deacetylase, UDP-(3-O-acyl)-N-acetylglucosamine deacetylase, UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase, UDP-3-O-(acyl)-N-acetylglucosamine deacetylase, UDP-3-O-(R-3-hydroxyacyl)-N-acetylglucosamine deacetylase, UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc deacetylase, UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase, UDP-3-O-(R-3-hydroxymyristoyl)-Nacetylglucosamine deacetylase, UDP-3-O-acyl-GlcNAc deacetylase, UDP-3-O-acyl-N-acetylglucosamine deacetylase, UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetylglucosamine amidohydrolase, UDP-3-O-[(R)-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase, UDP-3-O-[R-3-hydroxymyristoyl]-GlcNAc deacetylase, UDP-3-O-[R-3-hydroxymyristoyl]-GlcNAc deacetylase enzyme, uridine diphosphate-(3-O-(R-3-hydroxymyristoyl))-N-acetylglucosamine deacetylase, zinc deacetylase LpxC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.108 UDP-3-O-acyl-N-acetylglucosamine deacetylase

Cloned

Cloned on EC 3.5.1.108 - UDP-3-O-acyl-N-acetylglucosamine deacetylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C193A/DELTAD284-L294 variant of LpxC overexpressed in Escherichia coli
envA is cloned into an isopropyl-1-thio-beta-D-galactopyranoside-inducible T7-based expression system
-
Escherichia coli BL21 is used as host for expression of Strep-tagged LpxC protein
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Pseudomonas aeruginosa
-
gene envA or lpxC, functional overexpression of the first 299 amino acids out of 303 of enzyme mutant C40S in an Escherichia coli strain BL21(DE3) pLysS, and with its inhibitors in the acrB-deficient variant strain BL21(DE3) DELTAacrB pLysS or ARC5329
gene lpxC, recombinant expression of enzyme mutant LpxC C63A
-
overepression of LpxC variants in Escherichia coli
subcloned the Pseudomonas aeruginosa lpxC gene into the T7-based expression vector pET11a
the paLpxC (residues 1-299, C40S) is cloned from genomic DNA by polymerase chain reaction and inserted into pPW4 expression vectors. The recombinant paLpxC is overexpressed