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3.5.1.105: chitin disaccharide deacetylase

This is an abbreviated version!
For detailed information about chitin disaccharide deacetylase, go to the full flat file.

Word Map on EC 3.5.1.105

Reaction

N,N'-diacetylchitobiose
+
H2O
=
N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine
 +
acetate

Synonyms

carbohydrate esterase family 4 chitin oligosaccharide deacetylase, CE family 4 COD, CE-14 deacetylase, ChbG, chitin disaccharide deacetylase, chitin oligosaccharide deacetylase, chitooligosaccharide deacetylase, chitooligosaccharide deacetylase homolog, COD, codA, Dac, Dacph, deacetylase DA1, diacetylchitobiose deacetylase, GlcNAc2 deacetylase, N,N'-diacetylchitobiose deacetylase, NodB, NodB homology domain-containing protein, Pa-COD, PF0354, PGTG_04950, Ph-Dac, PH0499, polysaccharide deacetylase, Sb-COD, Sbal_1411, Tk-Dac, TK1764, VC_1280, Vp-COD, VP2638, YdjC, YDJC deacetylase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.105 chitin disaccharide deacetylase

Cloned

Cloned on EC 3.5.1.105 - chitin disaccharide deacetylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression of the soluble recombinant protein in Escherichia coli
gene chbG or ydjC, encoded in the chb operon, DNA and amino acid sequence determination and analysis
gene cod, recombinant expression of StrepII-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
-
gene nodB, recombinant expression of StrepII-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PH0499, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) and in Bacillus subtilis strain WB600, subcloning in Escherichia coli strain JM109, method optimization, overview
gene PH0499, recombinant expression of wild-type and mutants in Bacillus subtilis strain WB600 under control of the HpaII promoter and secreteion as extracellular enzymes, screening of signal peptides from Bacillus subtilis for optimal expression. The signal peptide YncM achieves the highest extracellular diacetylchitobiose deacetylase activity of 13.5 U/ml. Subcloning in Escherichia coli. Quantitative real-time PCR expression analysis. Method optimization, overview
gene Sbal_1411, cloning in Escherichia coli strain DH5alpha and recombinant expression in Escherichia coli strain HMS174(DE3), the recombinant protein is secreted to the culture medium
gene VP2638, quantitative real-time PCR expression analysis
overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys. The insoluble inclusion body is solubilized and reactivated through a refolding procedure
production of a recombinant chitin oligosaccharide deacetylase from Vibrio parahaemolyticus in the culture medium of Escherichia coli cells. The concentration of the recombinant enzyme in the Escherichia coli culture medium is 150times higher than that of wild-type enzyme produced in the culture medium by Vibrio parahaemolyticus KN1699
-
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)