3.4.25.2: HslU-HslV peptidase
This is an abbreviated version!
For detailed information about HslU-HslV peptidase, go to the full flat file.
Word Map on EC 3.4.25.2
Reaction
ATP-dependent cleavage of peptide bonds with broad specificity. =
Synonyms
AAA+ HslUV protease, AAA+ protease HslUV, ATP-dependent protease, ATP-dependent protease hslV, ClpQ, ClpQY, ClpYQ, ClpYQ complex, ClpYQ protease, CodW, CodW-CodX, heat shock protein hslV, HslU ATPase, HslU chaperone, HslU/HslV, HslUV, HslUV complex, HslUV protease, HslUV protease-chaperone complex, HslV, HslV peptidase, HslV protease, HslV-HslU, hslVU, HslVU ATP-dependent protease, HslVU protease, LINF_150005800, PfHslUV, T01.006
ECTree
3.4.25.2 HslU-HslV peptidaseAdvanced search results
results (
results (Crystallization
Crystallization on EC 3.4.25.2 - HslU-HslV peptidase
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
purified recombinant CodX and CodW and hybrid complexes with Escherichia coli enzymes CodW-HslU and HslV-CodX, X-ray diffraction strucure determination and analysis at 3.5-4.6 A resolution, the co-crystals contain lattice-translocation defects, correction, application of the lattice-translocation defect theory to atomic models, overview
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crystal structure of an 820000 Da relative molecular mass complex of the ATPase HslU and the protease component HslV, sitting drop vapour diffusion against a reservoir containing 100 mM sodium cacodylate pH 6.5, 15% glycerol, 10.5% polyethylene glycol PEG 8K and 500 mM (NH4)2SO4
hanging-drop vapor diffusion method. 3.0 A resolution crystal structure of hslV with an HslU hexamer bound at one end of an HslV dodecamer. The structure shows that the central pores of the ATPase and peptidase are next to each other and alligned
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mutant enzyme L88A, hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.0), 28% (v/v) PEG monomethyl ether 550, and 0.2 M ammonium formate
purified recombinant HslU and HslV and hybrid complexes with Bacillus subtilis enzymes CodW-HslU and HslV-CodX, X-ray diffraction strucure determination and analysis at 3.5-4.6 A resolution, the co-crystals contain lattice-translocation defects, correction, application of the lattice-translocation defect theory to atomic models, overview
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sitting drop vapor difussion against a reservoir containing 100 mM Hepes/NaOH at pH 7.5, 200 mM sodium acetate, 0.02% NaN3 and between 9% and 14% ethanol
the crystal strcuture shows that HslU forms a hexamer with a pore at one end and HslV forms a dodecamer with translocation pores at both ends of two back-to-back stacked hexameric rings
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wild-type or HslV-HsvU complexed with resorufin casein, hanging-drop vapor diffusion method
asymmetric HslU(DELTAI)6HslV12 complex.HslV can be activated by binding of a hexameric HslU(DELTAI)6 ring lacking the I domain
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