3.4.25.2: HslU-HslV peptidase
This is an abbreviated version!
For detailed information about HslU-HslV peptidase, go to the full flat file.
Word Map on EC 3.4.25.2
Reaction
ATP-dependent cleavage of peptide bonds with broad specificity. =
Synonyms
AAA+ HslUV protease, AAA+ protease HslUV, ATP-dependent protease, ATP-dependent protease hslV, ClpQ, ClpQY, ClpYQ, ClpYQ complex, ClpYQ protease, CodW, CodW-CodX, heat shock protein hslV, HslU ATPase, HslU chaperone, HslU/HslV, HslUV, HslUV complex, HslUV protease, HslUV protease-chaperone complex, HslV, HslV peptidase, HslV protease, HslV-HslU, hslVU, HslVU ATP-dependent protease, HslVU protease, LINF_150005800, PfHslUV, T01.006
ECTree
3.4.25.2 HslU-HslV peptidaseAdvanced search results
results (
results (Cloned
Cloned on EC 3.4.25.2 - HslU-HslV peptidase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression of His-tagged wild-type and mutant enzymes in BW25113 DELTAhslVU::kan cells
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expression of wild-type and mutant enzyme sin Saccharomyces cerevisiae strain EGY48 containing plasmid BD-sulA or BD-sulA mutant M89I
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gene clpQY or hslVU, expression of diverse gene constructs, e.g. as lacZ fusion constructs, and of truncated variants, in AC3112 cells, analysis of regulation of gene expression, overview. The stem-loop secondary structure of 5'-UTR of clpQ+Y+ is responsible for its mRNA stability
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gene clpY and gene clpQ, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli strain DH5alpha
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genes clpQ and clpY, DNA sequence determination, overexpression in a Lon protease-deficient mutant strain suppresses expression of the cps gene and of mucoid phenotype
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genes clpY, co-expression of ClpQ and ClpY mutants in AC3112 cells. Co-expression of ClpY with HA-tagged SulA and mutant SulA M89I, RcsA, RpoH, and TraJ molecules in the yeast two-hybrid system, expression of recombinant ClpYQ mutants
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HslU and HslV were coexpressed in BL21 (DE3) pLysS cells
mutant enzymes K80T, E286Q, E312Q, R325E, R393A, DELTA137-150, DELTA175-209, DELTA111-239, E266Q, Es66Q/E385K, I312W, ins(264,265), Ins(311, 312), Ins(387,388), Ins(435,436), DELTA432-443, E436A/D437A, E436K/D437K, E88Q, E88Q/E266Q, Y91G, V92G, G93A, E95W, DELTA88-92, DELTA89-92
the amplified fragment coding for HslV-EFHHHHHH is cloned into pET12b usind restriction sites Nde and SalI, expression in Escherichia coli
