Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.25.1: proteasome endopeptidase complex

This is an abbreviated version!
For detailed information about proteasome endopeptidase complex, go to the full flat file.

Word Map on EC 3.4.25.1

Reaction

cleavage of peptide bonds with very broad specificity =

Synonyms

20 S proteasome, 206 proteasome, 20S CP, 20S protease core, 20S proteasome, 26 S proteasome, 26S protease, 26S proteasome, 26S proteasome complex, 27 kDa prosomal protein, 30 kDa prosomal protein, beta1-PF1404, c20S, Component Y8, constitutive proteasome 20S, EC 3.4.22.21, EC 3.4.24.5, EC 3.4.99.46, GPRO-28, HsBPROS26, HSN3, i20S, immunoproteasome 20S, ingensin, KIPase, large multicatalytic protease, macropain, multi-subunit protease complex, multicatalytic endopeptidase complex, Multicatalytic endopeptidase complex C7, multicatalytic protease, multicatalytic proteinase, p27K, PROS-27, PROS-30, PROS-Dm25, PROS-Dm28.1, PROS-Dm29, PROS-Dm35, Pros26.4, prosome, proteasome, proteasome 19S, proteasome 20S, Proteasome component C13, Proteasome component C2, Proteasome component C3, Proteasome component C5, Proteasome component C8, Proteasome component C9, Proteasome component DD4, Proteasome component DD5, Proteasome component pts1, proteasome subunit alpha-type 7-like, PSMA5, Psma8, RING12 protein, RN3, Scl1, SCL1 suppressor protein, SFRICE_003436, T20S, TAS-F22/FAFP98, TAS-G64, TCPR29, tricorn protease, tricorn proteinase, XC3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.25 Threonine endopeptidases
                3.4.25.1 proteasome endopeptidase complex

Purification

Purification on EC 3.4.25.1 - proteasome endopeptidase complex

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
20S proteasome complex
-
ammonium sulfate precipitation, Q-Sepharose column chromatography, CHTII hydroxyapatite column chromatography, and phenyl-Sepharose column chromatograpyh
-
anti-Flag M2 agarose bead chromatography, and gel filtration
DEAE column chromatography, MonoQ HR5/5 column chromatography, and Superose 6 gel filtration
-
DEAE-Toyopearl 650S column chromatography and Superose 6 gel filtration
-
development of a method to purtify the 26S proteasome intact from whole Arabidopsis seedlings
-
development of an affinity method to rapidly purify intact epitope-tagged 26S proteasomes using mutant homozygous PAG1-FLAG pag1-1 line
-
development of an integrated proteomic approach, QTAX, for quantitative analysis of tandem affinity purified in vivo cross-linked protein complexes to capture protein interactions of all natures in a single analysis, overview. Investigation of cell cycle specific proteasome interaction networks. Wild-type and RPN11-HBH cells are synchronized in three phases (G1, S, and M) before cross-linking and tandem affinity purification using nickel affinity resin and streptavidin beads after lysis using buffer containing 8 M urea, 300 mM NaCl, 50 mM NaH2PO4, 0.5% Igepal, 20 mM imidazole, and 1 mM PMSF, pH 8.5
-
fungal 26S proteasome, comprising 102 distinct proteins, by anion exchange chromatography and gel filtration, method optimization, mass spectrometry and protein identifications, overview
-
glutathione Sepharose 4B beads chromatography
-
Hiload 16/60 Superdex 200 gel filtration
-
HiTrap phenyl-Sepharose column chromatography
IgG-affinity resin chromatography
-
native 20S proteasomes from hearts by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography
-
native 26S proteasomes from cytosolic or synaptosomal extracts of brain cortex by nickel and glutathione affinity chromatography
-
Ni2+ chelate chromatography
-
nickel affinity chromatography
-
partially by preparation of erythrocytes, lysis, and differential centrifugation, determination of the subunits in proteasomal analysis, overview
-
presence of 10% glycerol required
-
presence of glycerol and ATP are not essential for purification
A0A286S218; A0A286S1Z7; A0A286S200
Q Fast Flow column chromatography, Mono-Q column chromatography
-
Q-Sepharose HP chromatography
-
several forms of 20S proteasomes from extracts of Spodoptera frugiperda (Sf9) cells are separated using electrophoresis in a native polyacrylamide gel
Superose 6 gel filtration and Superdex 200 gel filtration
-