3.4.24.B39: metallo-alpha-fibrinogenase

This is an abbreviated version!
For detailed information about metallo-alpha-fibrinogenase, go to the full flat file.

Reaction

The enzymes cleaves the Aalpha-chain of fibrinogen with high specificity =

Synonyms

alpha-FG, alpha-Fibrinogenase, DAnase, fibrinogenase, rFII

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.B39 metallo-alpha-fibrinogenase

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Results	in table
1	AA Sequence
2	Application
2	General Information
12	Inhibitors
1	KM Value [mM]
2	Metals/Ions
2	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
5	Organism
2	pH Range
3	pI Value
1	Purification (Commentary)
1	Reaction
5	Reference
5	Source Tissue
16	Substrates and Products (Substrate)
2	Subunits
6	Synonyms
2	Temperature Range [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.4.24.B39 - metallo-alpha-fibrinogenase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

azocasein + H2O

?

Deinagkistrodon acutus

-

-

731371

-

-

?

Bovine fibrinogen + H2O

?

Deinagkistrodon acutus

A2TK72

the enzyme rapidly hydrolyzes the Aalpha-chain of fibrinogen, followed by the Bbeta-chain and does not cleave the gamma-chain

732876

-

-

?

casein + H2O

?

Deinagkistrodon acutus

-

-

731371

-

-

?

D-Pro-Phe-Arg-4-nitroanilide + H2O

D-Pro-Phe-Arg + 4-nitroaniline

Deinagkistrodon acutus

-

synthetic substrate P7959

731371

-

-

?

Fibrin + H2O

?

Deinagkistrodon acutus

-

-

731371

-

-

?

Fibrinogen + H2O

?

2 entries

Fibronectin + H2O

?

Deinagkistrodon acutus

-

-

731371

-

-

?

Human fibrinogen + H2O

?

Naja oxiana

-

the enzyme cleaves dose-dependently the Aalpha-chain of human fibrinogen only. The enzyme cleaves a peptide with molecular weight of around 10 kDa from the C-terminus

731649

-

-

?

N-(4-tosyl)-Gly-Pro-Lys-4-nitroanilide + H2O

N-(4-tosyl)-Gly-Pro-Lys + 4-nitroaniline

Deinagkistrodon acutus

-

best synthetic substrate, T6140

731371

-

-

?

N-benzoyl-Pro-Phe-Arg-4-nitroanilide + H2O

N-benzoyl-Pro-Phe-Arg + 4-nitroaniline

Deinagkistrodon acutus

-

synthetic substrate B2133

731371

-

-

?

Oxidized insulin B-chain + H2O

?

Deinagkistrodon acutus

-

the enzyme preferentially cleaves oxidized insulin B-chain at the site of Val12-Glu13, Leu15-Tyr16, and Phe24-Phe25

731371

-

-

?

plasmin + H2O

?

Deinagkistrodon acutus

-

-

731371

-

-

?

type IV collagen

?

Deinagkistrodon acutus

-

-

731371

-

-

?

additional information

?

-

3 entries

Fibrinogen + H2O

?

Deinagkistrodon acutus

-

the enzyme preferentially cleaves the Aalpha-chain of fibrinogen, followed by the Bbeta-chain and finally the gamma chain

731371

-

-

?

Fibrinogen + H2O

?

Echis multisquamatus

-

the enzyme predominantly cleaves the BbetaArg42-Ala43 peptide bond of fibrinogen, hydrolysis of its Aalpha-chain occurs much slower

755319

-

-

?

additional information

?

-

Deinagkistrodon acutus

A2TK72

the enzyme is devoid of fibrinolytic activity and also exhibits arginine esterase activity

732876

-

-

?

additional information

?

-

Deinagkistrodon acutus

-

the enzyme is devoid of fibrinolytic activity and also exhibits arginine esterase activity

732876

-

-

?

additional information

?

-

Echis multisquamatus

-

the BbetaArg42-Ala43 peptide bond is available for cleavage in all forms of fibrin(ogen) with the exception of polymerized fibrin desAB. The Aalpha-chain of fibrinogen remains intact after 3 min of incubation with fibrinogenase. Further incubation results in cleaving of the fibrin(ogen) alphaC-regions with the formation of two kinds of degradation products (about 30 and about 60 kDa)

755319

-

-

?