3.4.24.B30: aeropyrolysin

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For detailed information about aeropyrolysin, go to the full flat file.

Reaction

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.B30 aeropyrolysin

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Results	in table
1	Activating Compound
6	Inhibitors
2	Localization
1	Metals/Ions
2	Molecular Weight [Da]
10	Organic Solvent Stability
10	Organism
1	pH Optimum
1	pH Range
1	Purification (Commentary)
2	Reference
2	Source Tissue
8	Substrates and Products (Substrate)
2	Subunits
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

Inhibitors

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Inhibitors on EC 3.4.24.B30 - aeropyrolysin

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

CaCl2

Aeropyrum pernix

-

the maximal enzymatic activity is obtained at 110°C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100°C. Below 100°C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity

727497

diisopropyl fluorophosphate

Aeropyrum pernix

-

1 mM, 81% inhibition

727497

EDTA

Aeropyrum pernix

-

1 mM, complete loss of activity

727497

EGTA

Aeropyrum pernix

-

1 mM, 96% inhibition

727497

phenylmethylsulfonyl fluoride

Aeropyrum pernix

-

1 mM, 45% inhibition

727497

soybean trypsin

Aeropyrum pernix

-

1 mg/ml, 82% inhibition

727497

-