3.4.24.B24: L-alanine-D-glutamate endopeptidase
This is an abbreviated version!
For detailed information about L-alanine-D-glutamate endopeptidase, go to the full flat file.
Word Map on EC 3.4.24.B24
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3.4.24.B24
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bacteriophage
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endolysins
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phage
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peptidoglycans
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listeria
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lytic
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monocytogenes
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hydrolases
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n-acetylmuramoyl-l-alanine
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amidase
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carboxypeptidase
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lysed
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murein
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phage-encoded
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biotechnology
- 3.4.24.B24
- bacteriophage
- endolysins
- phage
- peptidoglycans
- listeria
-
lytic
- monocytogenes
- hydrolases
- n-acetylmuramoyl-l-alanine
- amidase
- carboxypeptidase
-
lysed
- murein
-
phage-encoded
- biotechnology
Reaction
endohydrolysis of the linkage of L-alanine-D-glutamic acid in the stem of a peptidoglycan =
Synonyms
CwlK, L,D-endopeptidase, L-alanoyl-D-glutamate endopeptidase, L-alanoyl-D-glutamate peptidase, Peptidoglycan hydrolase, peptidoglycan L-alanyl-D-glutamate endopeptidase cwIK, Ply500, TagX, YcdD
ECTree
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Cloned
Cloned on EC 3.4.24.B24 - L-alanine-D-glutamate endopeptidase
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into the pASK-IBA5 vector for expression of truncated Ply500 fragments, EAD500(1-141), EAD500(1-151), EAD500(1-163), in Escherichia coli JM83 cells
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truncated CwlK fused with a histidine-tag, h-deltaCwlK, is produced in Escherichia coli cells, fusions with the 3xFLAG-tag are constructed